Hormonal regulation of the Rous sarcoma virus src gene via a heterologous promoter defines a threshold dose for cellular transformation
- PMID: 6091899
- DOI: 10.1016/0092-8674(84)90271-x
Hormonal regulation of the Rous sarcoma virus src gene via a heterologous promoter defines a threshold dose for cellular transformation
Abstract
We have derived rat cell lines producing different and regulatable amounts of pp60v-src by introducing the src gene of Rous sarcoma virus (RSV) under the control of the glucocorticoid-responsive transcriptional promoter from the mouse mammary tumor virus (MMTV). We find that the cellular phenotype is strictly dependent upon the dose of pp60v-src with a distinct threshold for changes indicative of neoplastic potential. Cells with low constitutive levels of pp60v-src are not phenotypically distinguishable from cells without v-src, but as little as a 4-fold increment in pp60v-src produces morphological transformation and anchorage-independent growth. These properties of the transformed state are achieved at levels of pp60v-src far below levels found in an RSV-transformed cell line, without detectable increase in phosphorylation of the major cellular target for tyrosine phosphorylation.
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