doi: 10.1126/science.170.3962.1095.
Plasminogen: purification from human plasma by affinity chromatography
- PMID: 5475635
- DOI: 10.1126/science.170.3962.1095
Item in Clipboard
Plasminogen: purification from human plasma by affinity chromatography
Science.
.
Abstract
Plasminogen was prepared from human plasma by affinity chromatography on L-lysine-substituted Sepharose. Thirty milligrams of plasminogen, with a specific activity of 100 caseinolytic units (Committee on Thrombolytic Agents) per milligram of nitrogen, were obtained from 340 milliliters of plasma. This corresponds to over 200-fold purification from plasma. Disc-gel electrophoresis at pH 8.3 indicated seven distinct bands, all of which contained activity.
Similar articles
-
Affinity purification of plasminogen by radial-flow affinity chromatography.J Chromatogr. 1991 Feb 22;539(2):531-3. doi: 10.1016/s0021-9673(01)83965-0. J Chromatogr. 1991. PMID: 1904448
-
Ion exchange and affinity chromatography during the purification of human plasminogen on sepharose-L-lysine.Thromb Res. 1974 May;4(5):675-86. doi: 10.1016/0049-3848(74)90222-9. Thromb Res. 1974. PMID: 4209021 No abstract available.
-
The relevance of the structure of lysine bound to Sepharose for the affinity of rabbit plasminogen;Biochim Biophys Acta. 1975 Feb 27;379(2):504-11. doi: 10.1016/0005-2795(75)90157-9. Biochim Biophys Acta. 1975. PMID: 1122301
-
Studies on plasminogen. IX. Purification of human plasminogen from Cohn fraction 3 by affinity chromatography.Can J Biochem. 1971 Sep;49(9):1055-61. doi: 10.1139/o71-154. Can J Biochem. 1971. PMID: 5159592 No abstract available.
-
Purification of plasma protein.Haemostasis. 1988;18 Suppl 1:115-9. doi: 10.1159/000215846. Haemostasis. 1988. PMID: 3280421 Review.
Cited by
-
Molecular Interactions of Human Plasminogen with Fibronectin-binding Protein B (FnBPB), a Fibrinogen/Fibronectin-binding Protein from Staphylococcus aureus.J Biol Chem. 2016 Aug 26;291(35):18148-62. doi: 10.1074/jbc.M116.731125. Epub 2016 Jul 7. J Biol Chem. 2016. PMID: 27387503 Free PMC article.
-
Activation of pro-urokinase and plasminogen on human sarcoma cells: a proteolytic system with surface-bound reactants.J Cell Biol. 1989 May;108(5):1987-95. doi: 10.1083/jcb.108.5.1987. J Cell Biol. 1989. PMID: 2523891 Free PMC article.
-
Initiation of plasma prorenin activation by Hageman factor-dependent conversion of plasma prekallikrein to kallikrein.Proc Natl Acad Sci U S A. 1979 Nov;76(11):5914-8. doi: 10.1073/pnas.76.11.5914. Proc Natl Acad Sci U S A. 1979. PMID: 42905 Free PMC article.
-
Plasminogen- 125 I responses in dogs to a single injection of urokinase and typhoid vaccine and to vascular injury.J Clin Invest. 1972 Jun;51(6):1363-77. doi: 10.1172/JCI106932. J Clin Invest. 1972. PMID: 4623459 Free PMC article.
-
Engineered endothelium-mimicking antithrombotic surfaces via combination of nitric oxide-generation with fibrinolysis strategies.Bioact Mater. 2024 Sep 27;43:319-329. doi: 10.1016/j.bioactmat.2024.09.011. eCollection 2025 Jan. Bioact Mater. 2024. PMID: 39415940 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
