Full length and discrete partial reverse transcripts of globin and chorion mRNAs
- PMID: 47272
- DOI: 10.1016/0092-8674(75)90157-9
Full length and discrete partial reverse transcripts of globin and chorion mRNAs
Abstract
Rabbit globin mRNA was copied by AMV reverse transcriptase in the presence of various concentrations of deoxyribonucleotides (dNTPs). The cDNAs were analyzed by electrophoresis under denaturing conditions in formamide-polyacrylamide gels. Discrete size products were detected, ranging from 65 to 650 nucleotides-that is, up to the full length of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation of full-length transcripts and made the incomplete copies less abundant. Hybridization and nuclease digestion experiments indicated that the full-size product is indeed a complete transcript of globin mRNA. Similar results were obtained with chorion mRNAs. The possible usefulness of the discrete partial transcripts is discussed.
Similar articles
-
Characterization of synthetic rabbit globin DNA.Arch Biochem Biophys. 1973 Oct;158(2):494-502. doi: 10.1016/0003-9861(73)90541-9. Arch Biochem Biophys. 1973. PMID: 4131387 No abstract available.
-
In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).Proc Natl Acad Sci U S A. 1972 Jan;69(1):264-8. doi: 10.1073/pnas.69.1.264. Proc Natl Acad Sci U S A. 1972. PMID: 4333043 Free PMC article.
-
Synthesis of DNA complementary to cellular slime mold messenger RNA by reverse transcriptase.Biochemistry. 1974 Sep 10;13(19):3917-22. doi: 10.1021/bi00716a016. Biochemistry. 1974. PMID: 4137959 No abstract available.
-
Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts.Proc Natl Acad Sci U S A. 1973 Apr;70(4):1122-6. doi: 10.1073/pnas.70.4.1122. Proc Natl Acad Sci U S A. 1973. PMID: 4123930 Free PMC article.
-
Rapid nucleotide sequencing of DNA.Am J Hum Genet. 1979 Jan;31(1):19-28. Am J Hum Genet. 1979. PMID: 373426 Free PMC article. Review. No abstract available.
Cited by
-
Synthesis of long, representative DNA copies of the murine RNA tumor virus genome.J Virol. 1975 Jan;17(1):168-74. doi: 10.1128/JVI.17.1.168-174.1976. J Virol. 1975. PMID: 54441 Free PMC article.
-
The nucleotide sequence of the 5' untranslated region of human gamma-globin mRNA.Nucleic Acids Res. 1978 Oct;5(10):3515-22. doi: 10.1093/nar/5.10.3515. Nucleic Acids Res. 1978. PMID: 318162 Free PMC article.
-
Gene pioneers: Donald Brown and Thomas Maniatis win the 2012 Lasker~Koshland Special Achievement Award in Medical Science.J Clin Invest. 2012 Oct;122(10):3383-6. doi: 10.1172/jci66476. J Clin Invest. 2012. PMID: 23193577 Free PMC article. No abstract available.
-
Analysis of intracellular small RNAs of mouse hepatitis virus: evidence for discontinuous transcription.Virology. 1987 Feb;156(2):342-54. doi: 10.1016/0042-6822(87)90414-4. Virology. 1987. PMID: 3027983 Free PMC article.
-
Reverse transcriptase from avian myeloblastosis virus.J Virol. 1979 Feb;29(2):517-22. doi: 10.1128/JVI.29.2.517-522.1979. J Virol. 1979. PMID: 85721 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
