doi: 10.1073/pnas.71.8.3204.
Methylated messenger RNA synthesis in vitro by purified reovirus
- PMID: 4528644
- PMCID: PMC388651
- DOI: 10.1073/pnas.71.8.3204
Item in Clipboard
Methylated messenger RNA synthesis in vitro by purified reovirus
Proc Natl Acad Sci U S A.
1974 Aug.
Abstract
Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.
Similar articles
-
Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6. doi: 10.1073/pnas.72.1.362. Proc Natl Acad Sci U S A. 1975. PMID: 1054511 Free PMC article.
-
Transcription of reovirus RNA.Basic Life Sci. 1974;3:181-200. doi: 10.1007/978-1-4613-4529-9_15. Basic Life Sci. 1974. PMID: 4856694 No abstract available.
-
Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine.Cell. 1975 Oct;6(2):185-95. doi: 10.1016/0092-8674(75)90009-4. Cell. 1975. PMID: 1182800
-
Biochemical events in mRNA formation in mammalian cells.Symp Soc Dev Biol. 1976;(34):53-74. Symp Soc Dev Biol. 1976. PMID: 798344 Review. No abstract available.
-
The reovirus replicative cycle.Annu Rev Biochem. 1976;45:375-408. doi: 10.1146/annurev.bi.45.070176.002111. Annu Rev Biochem. 1976. PMID: 786154 Review.
Cited by
-
Nucleotide sequences at the 5' termini of reovirus mRNA's.J Virol. 1978 Nov;28(2):490-8. doi: 10.1128/JVI.28.2.490-498.1978. J Virol. 1978. PMID: 722859 Free PMC article.
-
Formation and characteristics of reovirus subviral particles in interferon-treated mouse L cells.Nucleic Acids Res. 1976 Mar;3(3):581-98. doi: 10.1093/nar/3.3.581. Nucleic Acids Res. 1976. PMID: 58408 Free PMC article.
-
"Pretranscriptional capping" in the biosynthesis of cytoplasmic polyhedrosis virus mRNA.Proc Natl Acad Sci U S A. 1978 Mar;75(3):1086-90. doi: 10.1073/pnas.75.3.1086. Proc Natl Acad Sci U S A. 1978. PMID: 349555 Free PMC article.
-
Investigating Viruses during the Transformation of Molecular Biology.J Biol Chem. 2017 Mar 10;292(10):3958-3969. doi: 10.1074/jbc.X117.778712. Epub 2017 Jan 30. J Biol Chem. 2017. PMID: 28154190 Free PMC article.
-
Reflections on the history of pre-mRNA processing and highlights of current knowledge: a unified picture.RNA. 2013 Apr;19(4):443-60. doi: 10.1261/rna.038596.113. Epub 2013 Feb 25. RNA. 2013. PMID: 23440351 Free PMC article. Review.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
