Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species
- PMID: 4355856
- PMCID: PMC356667
- DOI: 10.1128/JVI.12.3.594-599.1973
Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species
Abstract
Class a and class b 30 to 40S RNA subunits obtained by heat dissociation from the 60 to 70S RNA complex of avian tumor viruses were compared with several RNA standards by electrophoresis in formamide-polyacrylamide gels. Class a RNA was found to have a lower electrophoretic mobility and hence probably a higher molecular weight than class b RNA. The absolute molecular weight of class a and b RNA could not be determined with accuracy, because the relationship between logarithm of molecular weight and mobility of the RNA standards was not linear. The size of class a RNA fell into the range of 2.4 x 10(6) to 3.4 x 10(6) daltons and that of class b into the range of 2.2 x 10(6) to 2.9 x 10(6) daltons, depending on the standards used. The possible biological significance of this difference is discussed.
Similar articles
-
Avian tumor virus RNA: a comparison of three sarcoma viruses and their transformation-defective derivatives by oligonucleotide fingerprinting and DNA-RNA hybridization.Proc Natl Acad Sci U S A. 1973 Aug;70(8):2266-70. doi: 10.1073/pnas.70.8.2266. Proc Natl Acad Sci U S A. 1973. PMID: 4365699 Free PMC article.
-
Electrophoretic analysis of ribosomal and viral ribonucleic acids with a simple technique for slicing low-concentration polyacrylamide gels.Appl Microbiol. 1971 Oct;22(4):538-45. doi: 10.1128/am.22.4.538-545.1971. Appl Microbiol. 1971. PMID: 4331767 Free PMC article.
-
The 60-70S RNA of avian sarcoma and leukosis viruses--distribution of class a and b subunits.Bibl Haematol. 1973;39:462-73. doi: 10.1159/000427877. Bibl Haematol. 1973. PMID: 4360178 No abstract available.
-
DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA.Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336-40. doi: 10.1073/pnas.68.10.2336. Proc Natl Acad Sci U S A. 1971. PMID: 4332808 Free PMC article.
-
Properties and location of poly(A) in Rous sarcoma virus RNA.J Virol. 1974 Dec;14(6):1515-29. doi: 10.1128/JVI.14.6.1515-1529.1974. J Virol. 1974. PMID: 4372409 Free PMC article.
Cited by
-
Configurational variants of oncornavirus RNAs.J Virol. 1976 Sep;19(3):810-9. doi: 10.1128/JVI.19.3.810-819.1976. J Virol. 1976. PMID: 184300 Free PMC article.
-
Messenger activity of ribonucleic acid form yeast mitochondria.Biochem J. 1975 Sep;149(3):507-12. doi: 10.1042/bj1490507. Biochem J. 1975. PMID: 1106402 Free PMC article.
-
Partial purification and characterization of the messenger RNA for cell fibronectin.Nucleic Acids Res. 1979 Aug 10;6(11):3471-80. doi: 10.1093/nar/6.11.3471. Nucleic Acids Res. 1979. PMID: 573888 Free PMC article.
-
RNA of replication-defective strains of Rous sarcoma virus.Proc Natl Acad Sci U S A. 1975 Apr;72(4):1569-73. doi: 10.1073/pnas.72.4.1569. Proc Natl Acad Sci U S A. 1975. PMID: 165514 Free PMC article.
-
In vitro synthesis of full-length DNA transcripts of Rous sarcoma virus RNA by viral DNA polymerase.Proc Natl Acad Sci U S A. 1975 Dec;72(12):4895-9. doi: 10.1073/pnas.72.12.4895. Proc Natl Acad Sci U S A. 1975. PMID: 174081 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
