Purification of human monocytes on microexudate-coated surfaces
- PMID: 417139
Item in Clipboard
Purification of human monocytes on microexudate-coated surfaces
J Immunol.
1978 Apr.
Abstract
Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.
Similar articles
-
Human monocyte adherence: a primary effect of chemotactic factors on the monocyte to stimulate adherence to human endothelium.J Immunol. 1987 Mar 15;138(6):1762-71. J Immunol. 1987. PMID: 3819394
-
Measurement of adhesion and spreading kinetics of baby hamster kidney and hybridoma cells using an integrated optical method.Biotechnol Prog. 1994 Sep-Oct;10(5):520-4. doi: 10.1021/bp00029a010. Biotechnol Prog. 1994. PMID: 7765377
-
Purified canine monocytes fail to confer concanavalin-A responsiveness to accessory-cell-depleted lymphocytes.Exp Hematol. 1984 Jan;12(1):45-51. Exp Hematol. 1984. PMID: 6698128
-
Cellular activity of murine phagocytes isolated from peripheral blood by a discontinuous gradient.Folia Biol (Praha). 1998;44(3):117-21. Folia Biol (Praha). 1998. PMID: 10730853
-
Subfractionation of human blood monocyte subsets with Percoll.Exp Hematol. 1984 Nov;12(10):800-4. Exp Hematol. 1984. PMID: 6510481
Cited by
-
Long-term human peripheral blood monocyte cultures: establishment, metabolism and morphology of primary human monocyte-macrophage cell cultures.Immunology. 1979 Oct;38(2):401-11. Immunology. 1979. PMID: 389789 Free PMC article.
-
Comparative study of phagocytosis and intracellular bactericidal activity of human monocytes and polymorphonuclear cells. Application of fluorochrome and extracellular quenching technique.Inflammation. 1988 Feb;12(1):87-97. doi: 10.1007/BF00915894. Inflammation. 1988. PMID: 3366485
-
Alternative splicing of the human IgA Fc receptor CD89 in neutrophils and eosinophils.Biochem J. 1996 Sep 15;318 ( Pt 3)(Pt 3):771-7. doi: 10.1042/bj3180771. Biochem J. 1996. PMID: 8836118 Free PMC article.
-
Depression of monocyte and polymorphonuclear leukocyte oxidative metabolism and bactericidal capacity by influenza A virus.Infect Immun. 1982 Jan;35(1):350-5. doi: 10.1128/iai.35.1.350-355.1982. Infect Immun. 1982. PMID: 7054126 Free PMC article.
-
Soluble non-cross-linked peptidoglycan polymers stimulate monocyte-macrophage inflammatory functions.Infect Immun. 1985 Sep;49(3):731-41. doi: 10.1128/iai.49.3.731-741.1985. Infect Immun. 1985. PMID: 3875561 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources