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. 1985 Nov 25;13(22):8165-80.
doi: 10.1093/nar/13.22.8165.

Growth-dependent regulation of rRNA synthesis is mediated by a transcription initiation factor (TIF-IA)

D Buttgereit, G Pflugfelder, I Grummt
Free PMC article

Growth-dependent regulation of rRNA synthesis is mediated by a transcription initiation factor (TIF-IA)

D Buttgereit et al. Nucleic Acids Res. .
Free PMC article

Abstract

Mouse RNA polymerase I requires at least two chromatographically distinct transcription factors (designated TIF-IA and TIF-IB) to initiate transcription accurately and efficiently in vitro. In this paper we describe the partial purification of TIF-IA by a four-step fractionation procedure. The amount or activity of TIF-IA fluctuates in response to the physiological state of the cells. Extracts from quiescent cells are incapable of specific transcription and do not contain detectable levels of TIF-IA. Transcriptionally inactive extracts can be restored by the addition of TIF-IA preparations that have been highly purified from exponentially growing cells. During the fractionating procedure TIF-IA co-purifies with RNA polymerase I, suggesting that it is functionally associated with the transcribing enzyme. We suggest that only those enzyme molecules that are associated with TIF-IA are capable to interact with TIF-IB and to initiate transcription.

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