Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Nov;4(11):2793-800.
doi: 10.1002/j.1460-2075.1985.tb04005.x.

Epoxide hydrolase is a marker for the smooth endoplasmic reticulum in rat liver

M M Galteau, B Antoine, H Reggio

Epoxide hydrolase is a marker for the smooth endoplasmic reticulum in rat liver

M M Galteau et al. EMBO J. 1985 Nov.

Abstract

Epoxide hydrolase (EH, EC 3.3.2.3) was chosen as a potential marker for smooth endoplasmic reticulum, because this enzyme is inducible by drugs such as phenobarbital. The hypothesis was verified in rat liver using immunochemical and immunocytochemical techniques. Antibodies were raised to the purified protein. These antibodies were affinity purified using the enzyme immobilized on Sepharose Ultrogel. The specificity of the antibodies was assayed by immunoelectrotransfer (Western blot). The labelling of rat liver thin frozen sections with protein A-gold particles demonstrated that the antibodies specifically recognised smooth endoplasmic reticulum membranes. Rough endoplasmic reticulum, other intracellular organelles and plasma membrane were unlabelled.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Cell Biol. 1974 Apr;61(1):213-31 - PubMed
    1. Ann N Y Acad Sci. 1965 Mar 12;123:79-97 - PubMed
    1. J Cell Biol. 1974 Sep;62(3):717-45 - PubMed
    1. Biochim Biophys Acta. 1965 Nov 8;108(3):333-9 - PubMed
    1. J Histochem Cytochem. 1966 Mar;14(3):215-32 - PubMed

Publication types