Dissociated neurons regenerate into sciatic but not optic nerve explants in culture irrespective of neurotrophic factors
- PMID: 4032004
- PMCID: PMC6565336
- DOI: 10.1523/JNEUROSCI.05-09-02415.1985
Dissociated neurons regenerate into sciatic but not optic nerve explants in culture irrespective of neurotrophic factors
Abstract
Explants of adult or 10-day-old rat sciatic and optic nerves were implanted as "bridges" through a silicon grease seal in a three-compartment chamber culture system, leading from a narrow center chamber to two adjacent side chambers. Dissociated newborn rat sympathetic or sensory neurons were plated into the center chamber and grown in the presence of optimal concentrations of nerve growth factor (NGF). By light microscopy, nerve fibers were seen to grow out of the sciatic nerve explants in the side chambers after 2 to 3 weeks. Electron microscopy showed large numbers of axons present inside the sciatic nerves, irrespective of the presence and number of living Schwann cells. Besides their tendency to fasciculate, axons grew with high preference on Schwann cell membranes and the Schwann cell side of the basal lamina, a situation identical to in vivo regeneration. In contrast to the sciatic nerves, no axons could be found under any condition in the optic nerves. This result points to the existence of extremely poor, non-permissive substrate conditions in the differentiated optic nerves which cannot be overcome by the strong fiber outgrowth-promoting effects of NGF.
Similar articles
-
Regrowth of PNS axons through grafts of the optic nerve of the Browman-Wyse (BW) mutant rat.J Neurocytol. 1992 Jun;21(6):402-12. doi: 10.1007/BF01191505. J Neurocytol. 1992. PMID: 1403005
-
Optic axons regenerate into sciatic nerve isografts only in the presence of Schwann cells.Brain Res Bull. 1988 Feb;20(2):223-31. doi: 10.1016/0361-9230(88)90182-7. Brain Res Bull. 1988. PMID: 3370505
-
Ultrastructural studies of dorsal root axons regenerating through adult frog optic and sciatic nerves.Microsc Res Tech. 1999 Aug 15-Sep 1;46(4-5):310-8. doi: 10.1002/(SICI)1097-0029(19990815/01)46:4/5<310::AID-JEMT8>3.0.CO;2-Z. Microsc Res Tech. 1999. PMID: 10469467
-
In vitro studies on the control of nerve fiber growth by the extracellular matrix of the nervous system.J Physiol (Paris). 1987;82(4):258-70. J Physiol (Paris). 1987. PMID: 3332689 Review.
-
Applications of Proteomics to Nerve Regeneration Research.In: Alzate O, editor. Neuroproteomics. Boca Raton (FL): CRC Press/Taylor & Francis; 2010. Chapter 15. In: Alzate O, editor. Neuroproteomics. Boca Raton (FL): CRC Press/Taylor & Francis; 2010. Chapter 15. PMID: 21882439 Free Books & Documents. Review.
Cited by
-
A dual laminin/collagen receptor acts in peripheral nerve regeneration.Proc Natl Acad Sci U S A. 1990 Feb;87(4):1319-22. doi: 10.1073/pnas.87.4.1319. Proc Natl Acad Sci U S A. 1990. PMID: 2154740 Free PMC article.
-
Enhanced synthesis of brain-derived neurotrophic factor in the lesioned peripheral nerve: different mechanisms are responsible for the regulation of BDNF and NGF mRNA.J Cell Biol. 1992 Oct;119(1):45-54. doi: 10.1083/jcb.119.1.45. J Cell Biol. 1992. PMID: 1527172 Free PMC article.
-
White matter of the CNS supports or inhibits neurite outgrowth in vitro depending on geometry.J Neurosci. 1999 Oct 1;19(19):8358-66. doi: 10.1523/JNEUROSCI.19-19-08358.1999. J Neurosci. 1999. PMID: 10493737 Free PMC article.
-
Axonal regeneration in zebrafish spinal cord.Regeneration (Oxf). 2018 Apr 22;5(1):43-60. doi: 10.1002/reg2.99. eCollection 2018 Mar. Regeneration (Oxf). 2018. PMID: 29721326 Free PMC article. Review.
-
A metalloprotease activity from C6 glioma cells inactivates the myelin-associated neurite growth inhibitors and can be neutralized by antibodies.Br J Cancer. 1998 Dec;78(12):1564-72. doi: 10.1038/bjc.1998.724. Br J Cancer. 1998. PMID: 9862565 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
