Abundant nuclear ribonucleoprotein form of CAD RNA
- PMID: 3990684
- PMCID: PMC366750
- DOI: 10.1128/mcb.5.3.569-575.1985
Abundant nuclear ribonucleoprotein form of CAD RNA
Abstract
Transcripts of the CAD gene in Syrian hamster cells are as abundant in the nucleus as in the cytoplasm. This was shown by in situ hybridization of whole cells and by solution and blot hybridization of subcellular fractions. Similar results were obtained both for wild-type cells and for a mutant containing amplified CAD genes in which the level of CAD RNA is 150-fold greater. CAD nuclear RNA is indistinguishable from mature mRNA by gel electrophoresis and blot hybridization. Discrete higher-molecular-weight precursors are undetectable, although the persistence of a short length of intervening sequence in the otherwise fully processed RNA is not excluded. CAD RNA is released from nuclei by sonication in physiological conditions in a ribonucleoprotein form that sediments as a broad peak at about 200S in a sucrose gradient. CAD sequences extracted from nuclei by treatment with EDTA and RNase are found in the 30S particles previously described.
Similar articles
-
U1, U2, and U6 small nuclear ribonucleoproteins (snRNPs) are associated with large nuclear RNP particles containing transcripts of an amplified gene in vivo.Proc Natl Acad Sci U S A. 1986 Sep;83(18):6721-5. doi: 10.1073/pnas.83.18.6721. Proc Natl Acad Sci U S A. 1986. PMID: 2944114 Free PMC article.
-
Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.Mol Cell Biol. 1983 Nov;3(11):2066-75. doi: 10.1128/mcb.3.11.2066-2075.1983. Mol Cell Biol. 1983. PMID: 6318080 Free PMC article.
-
Start site selection at the TATA-less carbamoyl-phosphate synthase (glutamine-hydrolyzing)/aspartate carbamoyltransferase/dihydroorotase promoter.J Biol Chem. 1994 Jan 21;269(3):2252-7. J Biol Chem. 1994. PMID: 7905000
-
[Gene amplification].Tanpakushitsu Kakusan Koso. 1984 Mar;29(3):152-63. Tanpakushitsu Kakusan Koso. 1984. PMID: 6425988 Review. Japanese. No abstract available.
-
CAD gene sequence and the domain structure of the mammalian multifunctional protein CAD.Biochem Soc Trans. 1993 Feb;21(1):186-91. doi: 10.1042/bst0210186. Biochem Soc Trans. 1993. PMID: 8095469 Review. No abstract available.
Cited by
-
A novel splicing factor is an integral component of 200S large nuclear ribonucleoprotein (InRNP) particles.EMBO J. 1991 Feb;10(2):425-32. doi: 10.1002/j.1460-2075.1991.tb07964.x. EMBO J. 1991. PMID: 1991453 Free PMC article.
-
Identification of a new promoter upstream of the murine dihydrofolate reductase gene.Mol Cell Biol. 1989 Oct;9(10):4568-70. doi: 10.1128/mcb.9.10.4568-4570.1989. Mol Cell Biol. 1989. PMID: 2479829 Free PMC article.
-
Identification of nuclear RNP proteins as pre-mRNA splicing factors.Mol Biol Rep. 1990;14(2-3):193. doi: 10.1007/BF00360469. Mol Biol Rep. 1990. PMID: 2141908 No abstract available.
-
Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA.J Biol Chem. 2009 May 22;284(21):14303-15. doi: 10.1074/jbc.M901026200. Epub 2009 Mar 12. J Biol Chem. 2009. PMID: 19282290 Free PMC article.
-
Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.Nucleic Acids Res. 2007;35(12):3928-44. doi: 10.1093/nar/gkm347. Epub 2007 May 30. Nucleic Acids Res. 2007. PMID: 17537823 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
