Clinical Trial
doi: 10.1016/0889-1605(85)90077-1.
Mica sandwich technique for preparing macromolecules for rotary shadowing
- PMID: 3903184
- DOI: 10.1016/0889-1605(85)90077-1
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Clinical Trial
Mica sandwich technique for preparing macromolecules for rotary shadowing
J Ultrastruct Res.
1985 Apr.
Abstract
The sandwich technique, in which a drop of sample solution is spread into a thin layer between two pieces of freshly cleaved mica, is a simple-to-use alternative to spraying for depositing macromolecules onto mica. Test specimens of collagen molecules and actin filaments were found to suffer less shear-induced damage, they were more uniformly distributed, and only very small sample volumes were needed. Either drying from a glycerol solution (40-70% v/v) or freeze-drying can be employed. Glycerol-drying is simpler, but freeze-drying may offer better preservation of supra-molecular assemblies.
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