Comparative Study
doi: 10.1016/s0092-8674(85)80057-x.
Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins
- PMID: 3891097
- DOI: 10.1016/s0092-8674(85)80057-x
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Comparative Study
Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins
Cell.
1985 Jul.
Abstract
In these experiments we demonstrate that purified RAS proteins, whether derived from the yeast RAS1 or RAS2 or the human H-ras genes, activate yeast adenylate cyclase in the presence of guanine nucleotides. These results confirm the prediction of earlier genetic and biochemical data and for the first time provide a complete biochemical assay for RAS protein function. Furthermore, we observe a biochemical difference between the RAS2 and RAS2val19 proteins in their ability to activate adenylate cyclase after preincubation with GTP.
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