Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity
- PMID: 389
Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity
Abstract
Heterogeneities of the two ovalbumin glycopeptides, (Man)5(GlcNAc)2Asn and (Man)6(GlcNAc)2Asn, were revealed by borate paper electrophoresis of oligosaccharide alcohols obtained from the glycopeptides by endo-beta-N-acetylglucosaminidase H digestion and NaB3H4 reduction. The structures of the major components of the oligosaccharides were determined by the combination of methylation analysis, acetolysis, and alpha-mannosidase digestion. Based on the results, the whole structures of the major components of (Man)5(GlcNAc)2Asn and (Man)6(GlcNAc)2Asn were elucidated as Manalpha1 leads to 6[Manalpha1 leads to 3]-Manalpha1 leads to 6[Manalpha1 leads to 3[Manbeta1 leads to 4GlcNAcbeta1 leads to 4GlcNAc leads to Asn and Manalpha1 leads to 6[Manalpha1 leads to 3]Manalpha1 leads to 6[Manalpha1 leads to 2Manalpha1 leads to 3]Manbeta1 leads to 4GlcNAcbeta1 leads to GlcNAc leads to Asn, respectively. Since endo-beta-N-acetylglucosamini dase D hydrolyzes (Man)5(GlcNAc)2Asn but not (Man)6(GlcNAc)2Asn, the presence of the unsubstituted alpha-mannosyl residue linked at the C-3 position of the terminal mannose of Manbeta1 leads to 4GlcNAcbeta1 leads to 4 GlcNAcAsn core must be essential for the action of the enzyme.
Similar articles
-
Asparaginyl glycopeptides with a low mannose content are hydrolyzed by endo-beta-N-acetylglucosaminidase H.J Biol Chem. 1978 Jul 10;253(13):4508-11. J Biol Chem. 1978. PMID: 659430
-
Structural studies of the major high mannose oligosaccharide units from Chinese hamster ovary cell glycoproteins.J Biol Chem. 1979 Mar 10;254(5):1600-5. J Biol Chem. 1979. PMID: 762159
-
Concanavalin A binding and endoglycosidase D resistance of beta1,2-xylosylated and alpha1,3-fucosylated plant and insect oligosaccharides.Glycoconj J. 1998 Feb;15(2):203-6. doi: 10.1023/a:1006932725821. Glycoconj J. 1998. PMID: 9557883
-
Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase.Biochem J. 2003 May 15;372(Pt 1):53-64. doi: 10.1042/BJ20021931. Biochem J. 2003. PMID: 12603202 Free PMC article.
-
Complex N-glycans: the story of the "yellow brick road".Glycoconj J. 2014 Jan;31(1):1-5. doi: 10.1007/s10719-013-9507-5. Epub 2013 Nov 2. Glycoconj J. 2014. PMID: 24178944 Review.
Cited by
-
Structural analysis of N-glycans from gull egg white glycoproteins and egg yolk IgG.Glycobiology. 2009 Jul;19(7):693-706. doi: 10.1093/glycob/cwp025. Epub 2009 Feb 24. Glycobiology. 2009. PMID: 19240272 Free PMC article.
-
The structure of carbohydrate unit B of porcine thyroglobulin.Biochem J. 1981 Jun 1;195(3):701-13. doi: 10.1042/bj1950701. Biochem J. 1981. PMID: 7316980 Free PMC article.
-
Retention of secretory proteins in an intermediate compartment and disappearance of the Golgi complex in an END4 mutant of Chinese hamster ovary cells.J Cell Biol. 1992 May;117(4):701-15. doi: 10.1083/jcb.117.4.701. J Cell Biol. 1992. PMID: 1577851 Free PMC article.
-
Glycosylation provides both stimulatory and inhibitory effects on cell surface and soluble CD44 binding to hyaluronan.J Cell Biol. 1998 Jan 26;140(2):431-46. doi: 10.1083/jcb.140.2.431. J Cell Biol. 1998. PMID: 9442118 Free PMC article.
-
Characterization of lipid-linked octa- through undecasaccharides implicated in the biosynthesis of Saccharomyces cerevisiae mannoproteins.J Bacteriol. 1983 Feb;153(2):895-902. doi: 10.1128/jb.153.2.895-902.1983. J Bacteriol. 1983. PMID: 6337129 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
