doi: 10.1016/0092-8674(85)90066-2.
mRNA splicing efficiency in yeast and the contribution of nonconserved sequences
- PMID: 3888403
- DOI: 10.1016/0092-8674(85)90066-2
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mRNA splicing efficiency in yeast and the contribution of nonconserved sequences
Cell.
1985 May.
Abstract
A simple kinetic model for mRNA splicing predicts the way in which in vivo steady state precursor RNA levels (P) and messenger RNA levels (M) vary as a function of the rate constant of the splicing reaction (ksp). The model points to M/P as the best measure of ksp. The analysis of a set of intron mutations in a yeast gene supports the general features of the model and shows that the splicing efficiency of transcripts containing the wild-type intron is well in excess of what is necessary to generate normal mRNA levels. The data also suggest that regions of the intron, in addition to the well-conserved consensus sequences, contribute to efficient splicing.
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