Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes
- PMID: 3874868
Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes
Abstract
Cytolytic granules from a T-cell line with specific cytolytic activity were isolated. Granules were solubilized and fractionated on a TSK 4000 gel filtration column. Lytic activity was eluted as a single retarded peak. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate indicated that the lytic fractions contained a single protein (perforin) with an apparent molecular weight of approximately 66 kDa. It separated well from the other proteins present in the granules. Isolated perforin polymerized and inserted into lipid bilayers in the presence of Ca2+, forming tubular structures with inner diameters varying from 6 to 16 nm. Lipid insertion of perforin was demonstrated using a membrane-restricted, photoactivatable probe. The lytic properties of perforin suggest an important role of this particular protein during cytolytic T-cell-mediated target cell lysis.
Similar articles
-
Isolation and biochemical and functional characterization of perforin 1 from cytolytic T-cell granules.Proc Natl Acad Sci U S A. 1985 Dec;82(24):8629-33. doi: 10.1073/pnas.82.24.8629. Proc Natl Acad Sci U S A. 1985. PMID: 2417226 Free PMC article.
-
Cytolytic T cell granules. Isolation, structural, biochemical, and functional characterization.J Exp Med. 1984 Sep 1;160(3):695-710. doi: 10.1084/jem.160.3.695. J Exp Med. 1984. PMID: 6332169 Free PMC article.
-
Interaction of chondroitin sulfate with perforin and granzymes of cytolytic T-cells is dependent on pH.Biochemistry. 1990 Dec 25;29(51):11229-35. doi: 10.1021/bi00503a011. Biochemistry. 1990. PMID: 2125503
-
Involvement of granule proteins in T-cell-mediated cytolysis.Nat Immun Cell Growth Regul. 1990;9(4):274-82. Nat Immun Cell Growth Regul. 1990. PMID: 2215515 Review.
-
Perforin-mediated target cell lysis by cytolytic T lymphocytes.Annu Rev Immunol. 1990;8:279-302. doi: 10.1146/annurev.iy.08.040190.001431. Annu Rev Immunol. 1990. PMID: 2188665 Review. No abstract available.
Cited by
-
Perforin activates clathrin- and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis.Blood. 2010 Feb 25;115(8):1582-93. doi: 10.1182/blood-2009-10-246116. Epub 2009 Dec 28. Blood. 2010. PMID: 20038786 Free PMC article.
-
Ultrastructural evidence that the granules of human natural killer cell clones store membrane in a nonbilayer phase.Am J Pathol. 1987 May;127(2):305-16. Am J Pathol. 1987. PMID: 3107394 Free PMC article.
-
Perforin expression in lymphocytes infiltrated to human colorectal cancer.Br J Cancer. 1991 Aug;64(2):239-42. doi: 10.1038/bjc.1991.283. Br J Cancer. 1991. PMID: 1892750 Free PMC article.
-
Dissociation of membrane binding and lytic activities of the lymphocyte pore-forming protein (perforin).J Exp Med. 1987 May 1;165(5):1371-82. doi: 10.1084/jem.165.5.1371. J Exp Med. 1987. PMID: 3494808 Free PMC article.
-
High activity of N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester serine esterase and cytolytic perforin in cloned cell lines is not demonstrable in in-vivo-induced cytotoxic effector cells.Proc Natl Acad Sci U S A. 1987 Jul;84(14):5004-8. doi: 10.1073/pnas.84.14.5004. Proc Natl Acad Sci U S A. 1987. PMID: 2955414 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
