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. 2024 Apr 18:16:175-187.
doi: 10.2147/JEP.S453578. eCollection 2024.

Evaluation of Diuretic Activity of Aqueous and Hydro Methanolic Crude Extracts and Solvent Fraction of the Hydromethanolic Flower Extract of Erica Arborea L. (Ericaceae) in Swiss Albino Mice

Nardos Lema Wondimu #  1 , Mestayet Geta Mengistie #  2 , Jibril Seid Yesuf #  2
Affiliations

Evaluation of Diuretic Activity of Aqueous and Hydro Methanolic Crude Extracts and Solvent Fraction of the Hydromethanolic Flower Extract of Erica Arborea L. (Ericaceae) in Swiss Albino Mice

Nardos Lema Wondimu et al. J Exp Pharmacol. .

Abstract

Purpose: To evaluate the diuretic effects of aqueous (AQ) and hydromethanolic crude extract (HM) the as well as the solvent fractions of the HM extract from Erica arborea flowers in mice.

Methods: Mice were administered AQ and HM crude extracts, along with solvent fractions of HM extracts of E. arborea flowers, including HXF (n-hexane fraction), EAF (ethyl acetate fraction), and AQF (aqueous fraction), at doses ranging from 100 to 400 mg/kg orally. The effects of these extracts and solvent fractions on urine and salt excretion over 5 hours were compared to the effects of the solvent used for reconstitution and a standard drug (furosemide 10 mg/kg), as well as to each other.

Results: The HM crude extract at a lower dose (100 mg/kg) significantly increased urine volume and salt excretion starting from the 3rd h compared to the AQ crude extract. Similar effects were observed for EAF. Notably, the HM extract and its EAF at 400 mg/kg showed comparable urine and salt excretion profiles to the standard drug.

Conclusion: This study demonstrated that HM extract and EAF promote better diuresis, likely due to their saluretic properties. Furthermore, it confirms the diuretic activity of Erica arborea flowers.

Keywords: diuresis; mice; natriuresis; urine electrolyte; urine pH.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Photographs of Erica arborea. L at its habitat.
Figure 2
Figure 2
Effect of AQ and HM flower extract of Erica arborea on urine pH in mice.
Figure 3
Figure 3
Effect of HM solvent fractions of flower of Erica arborea on urine pH in mice.
Figure 4
Figure 4
Electrolyte content of flower of Erica arborea (mmol/l).
See this image and copyright information in PMC

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References

    1. Skourtis D, Stavroulaki D, Athanasiou V, Fragouli PG, Iatrou H. Nanostructured polymeric, liposomal and other materials to control the drug delivery for cardiovascular diseases. Pharmaceutics. 2020;12(12):1160. doi:10.3390/pharmaceutics12121160 - DOI - PMC - PubMed
    1. Groenewegen A, Rutten FH, Mosterd A, Hoes AW. Epidemiology of heart failure. Eur J Heart Fail. 2020;22(8):1342–1356. doi:10.1002/ejhf.1858 - DOI - PMC - PubMed
    1. Pellicori P, Platz E, Dauw J, et al. Ultrasound imaging of congestion in heart failure: examinations beyond the heart. Eur J Heart Fail. 2021;23(5):703–712. doi:10.1002/ejhf.2032 - DOI - PMC - PubMed
    1. Smith H. Diuretics: a review for the pharmacist. Saudi Pharm J. 2014;81(7):18–21.
    1. Kehrenberg MC, Bachmann HS. Diuretics: a contemporary pharmacological classification? Naunyn-Schmiedeberg’s Arch Pharmacol. 2022;395(6):619–627. doi:10.1007/s00210-022-02228-0 - DOI - PMC - PubMed

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