Initiator tRNAs have a unique anticodon loop conformation
- PMID: 386336
- PMCID: PMC383810
- DOI: 10.1073/pnas.76.7.3289
Initiator tRNAs have a unique anticodon loop conformation
Abstract
Transfer RNA (tRNA) molecules have been labeled with 32P at the 5' end and subjected to S1 nuclease digestion. The products were analyzed by high-resolution gel electrophoresis. Three initiator tRNAs and six chain-elongating tRNAs were examined. S1 nuclease cleaved Escherichia coli tRNAfMet, yeast tRNAfMet, and mammalian tRNAfMet at the same two positions in the anticodon loop. In contrast, S1 nuclease cleaved the anticodon loop of E. coli tRNAmMet, yeast tRNAmMet, yeast tRNAPhe, Schizosaccharomyces pombe tRNAPhe, E. coli tRNA2Glu, and E. coli tRNATrp (su+) at four positions generally, except where a modified nucleotide in the wobble position inhibited the enzyme. The marked contrast between these cleavage patterns suggests a different conformation for the anticodon loops of these two classes of tRNA molecules. It is suggested that the specialized conformation in the anticodon loop of initiator tRNAs may be due to a special sequence of GC base pairs in the adjoining anticodon stem.
Similar articles
-
Stability of the unique anticodon loop conformation of E.coli tRNAfMet.Nucleic Acids Res. 1979 Nov 24;7(6):1457-67. doi: 10.1093/nar/7.6.1457. Nucleic Acids Res. 1979. PMID: 41223 Free PMC article.
-
Role of the three consecutive G:C base pairs conserved in the anticodon stem of initiator tRNAs in initiation of protein synthesis in Escherichia coli.RNA. 1996 May;2(5):473-82. RNA. 1996. PMID: 8665414 Free PMC article.
-
A unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis.Nucleic Acids Res. 2008 Sep;36(15):4894-901. doi: 10.1093/nar/gkn462. Epub 2008 Jul 24. Nucleic Acids Res. 2008. PMID: 18653533 Free PMC article.
-
Escherichia coli initiator tRNA: structure-function relationships and interactions with the translational machinery.Biochem Cell Biol. 1995 Nov-Dec;73(11-12):1023-31. doi: 10.1139/o95-109. Biochem Cell Biol. 1995. PMID: 8722017 Review.
-
Recognition in nucleic acids and the anticodon families.Prog Nucleic Acid Res Mol Biol. 1973;13:301-37. doi: 10.1016/s0079-6603(08)60106-3. Prog Nucleic Acid Res Mol Biol. 1973. PMID: 4573490 Review. No abstract available.
Cited by
-
The tRNA-like structure of turnip yellow mosaic virus RNA: structural organization of the last 159 nucleotides from the 3' OH terminus.EMBO J. 1982;1(2):269-76. doi: 10.1002/j.1460-2075.1982.tb01158.x. EMBO J. 1982. PMID: 16453415 Free PMC article.
-
The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons.J Mol Biol. 2011 Feb 18;406(2):257-74. doi: 10.1016/j.jmb.2010.11.042. Epub 2010 Dec 17. J Mol Biol. 2011. PMID: 21168417 Free PMC article.
-
Nucleotide sequence of cytoplasmic initiator tRNA from Tetrahymena thermophila.Nucleic Acids Res. 1981 Sep 25;9(18):4557-62. doi: 10.1093/nar/9.18.4557. Nucleic Acids Res. 1981. PMID: 7301582 Free PMC article.
-
Secondary structure of the circular form of the Tetrahymena rRNA intervening sequence: a technique for RNA structure analysis using chemical probes and reverse transcriptase.Proc Natl Acad Sci U S A. 1985 Feb;82(3):648-52. doi: 10.1073/pnas.82.3.648. Proc Natl Acad Sci U S A. 1985. PMID: 2579378 Free PMC article.
-
A functional analysis of the repeated methionine initiator tRNA genes (IMT) in yeast.Mol Gen Genet. 1989 Apr;216(2-3):276-86. doi: 10.1007/BF00334366. Mol Gen Genet. 1989. PMID: 2664453
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
