Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jul;6(7):2721-6.
doi: 10.1128/mcb.6.7.2721-2726.1986.

Tropomyosin isoform switching in tumorigenic human fibroblasts

J Leavitt, G Latter, L Lutomski, D Goldstein, S Burbeck

Tropomyosin isoform switching in tumorigenic human fibroblasts

J Leavitt et al. Mol Cell Biol. 1986 Jul.

Abstract

We identified six tropomyosin (Tm) isoforms in diploid human fibroblasts. We used computerized microdensitometry of 2-dimensional protein profiles to measure the relative rates of synthesis and abundance of the individual Tm isoforms and actin, the two major structural constituents of microfilaments. In carcinogen-transformed human fibroblasts (HuT-14), the rates of synthesis of three Tm isoforms (Tm1, Tm2, and Tm6) were greatly decreased relative to normal diploid parental fibroblasts and to actin. In contrast, related nontumorigenic HuT fibroblasts which are "immortalized" and anchorage independent exhibited both slight down-regulation of Tm1 and Tm6 and 3.5-fold up-regulation of Tm3. Thus, Tm isoform switching from the predominance of the larger more avid Tm isoforms (Tm1, Tm2, Tm3, and Tm6) to the smaller, less avid Tm isoforms (Tm4 and Tm5) in microfilaments was a transformation-induced change correlated with tumorigenicity in human fibroblasts.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 1978 Feb 25;253(4):1137-48 - PubMed
    1. Ann N Y Acad Sci. 1986;468:339-49 - PubMed
    1. J Biol Chem. 1979 Aug 25;254(16):7961-77 - PubMed
    1. Differentiation. 1979;14(3):123-33 - PubMed
    1. Dev Biol. 1979 Nov;73(1):134-52 - PubMed

Publication types

LinkOut - more resources