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. 1979 May;76(5):2222-6.
doi: 10.1073/pnas.76.5.2222.

Cloning in Escherichia coli and physical structure of hepatitis B virion DNA

P Charnay, C Pourcel, A Louise, A Fritsch, P Tiollais

Cloning in Escherichia coli and physical structure of hepatitis B virion DNA

P Charnay et al. Proc Natl Acad Sci U S A. 1979 May.

Abstract

A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.

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