Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Mar;137(3):1185-90.
doi: 10.1128/jb.137.3.1185-1190.1979.

Synthesis and modification of proteins during the cell cycle of the yeast Saccharomyces cerevisiae

S G Elliott, C S McLaughlin

Synthesis and modification of proteins during the cell cycle of the yeast Saccharomyces cerevisiae

S G Elliott et al. J Bacteriol. 1979 Mar.

Abstract

We have used a novel technique to study the synthesis, modification and degradation of proteins during the cell cycle in Saccharomyces cerevisiae. Logarithmically growing cells were pulse-labeled twice, with the pulses separated in time by more than one generation. Subsequently, the cells were fractionated as to their position in the cell cycle by centrifugal elutriation, and for different proteins the ratio of radioactive material from the two pulses was then determined. Periodic degradation, synthesis, or modification would produce periodic variations in the ratio of counts. Two-dimensional gel electrophoresis was used to examine 110 different proteins at different times of the cell cycle. All but two proteins had a constant ratio of counts through the cell cycle. This indicates that the rate of synthesis of individual proteins increases exponentially during the cell cycle and that periodic degradation or modification of proteins is not a general feature of the cell cycle in S. cerevisiae.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
    1. J Bacteriol. 1977 Jan;129(1):97-100 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4384-8 - PubMed
    1. Methods Cell Biol. 1977;15:1-14 - PubMed
    1. J Bacteriol. 1976 Mar;125(3):887-91 - PubMed

Publication types

Substances

LinkOut - more resources