doi: 10.1016/0003-2697(86)90176-4.
Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions
- PMID: 3740412
- DOI: 10.1016/0003-2697(86)90176-4
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Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions
Anal Biochem.
1986 Jul.
Abstract
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.
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