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. 2023 Mar 27;23(2):103.
doi: 10.1007/s10142-023-01033-z.

Genome survey sequencing-based SSR marker development and their validation in Dendrocalamus longispathus

Rajendra K Meena  1 , Priyanka Kashyap  2 , Arzoo Shamoon  2 , Payal Dhyani  2 , Hansraj Sharma  3   4 , Maneesh S Bhandari  2 , Santan Barthwal  2 , Harish S Ginwal  2
Affiliations

Genome survey sequencing-based SSR marker development and their validation in Dendrocalamus longispathus

Rajendra K Meena et al. Funct Integr Genomics. .

Abstract

Bamboo is an important genetic resource in India, supporting rural livelihood and industries. Unfortunately, most Indian bamboo taxa are devoid of basic genomic or marker information required to comprehend the genetic processes for further conservation and management. In this study, we perform genome survey sequencing for development of de novo genomic SSRs in Dendrocalamus longispathus, a socioeconomically important bamboo species of northeast India. Using Illumina platform, 69.49 million raw reads were generated and assembled into 1,145,321 contig with GC content 43% and N50 1228 bp. In total, 46,984 microsatellite repeats were mined-out wherein di-nucleotide repeats were most abundant (54.71%) followed by mono- (31.91%) and tri-repeats (9.85%). Overall, AT-rich repeats were predominant in the genome, but GC-rich motifs were more frequent in tri-repeats. Afterwards, 21,596 SSR loci were successfully tagged with the primer pairs, and a subset of 50 were validated through polymerase chain reaction amplification. Of these, 36 SSR loci were successfully amplified, and 16 demonstrated polymorphism. Using 13 polymorphic SSRs, a moderate level of gene diversity (He = 0.480; Ar = 3.52) was recorded in the analysed populations of D. longispathus. Despite the high gene flow (Nm = 4.928) and low genetic differentiation (FST = 0.119), severe inbreeding (FIS = 0.407) was detected. Further, genetic clustering and STRUCTURE analysis revealed that the entire genetic variability is captured under two major gene pools. Conclusively, we present a comprehensive set of novel SSR markers in D. longispathus as well as other taxa of tropical woody bamboos.

Keywords: Bamboo; Cross amplification; GSS; Genetic diversity; Population genetic analysis; SSR markers.

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References

    1. Abreu AG, Grombone-Guaratini MT, Monteiro M, Pinheiro JB, Tombolato AFC, Zucchi MI (2011) Development of microsatellite markers for Aulonemia aristulata (Poaceae) and cross-amplification in other bamboo species. Am J Bot 98:90–92. https://doi.org/10.3732/ajb.1000511 - DOI
    1. Adhikari S, Saha S, Biswas A, Rana TS, Bandyopadhyay TK, Ghosh P (2017) Application of molecular markers in plant genome analysis: a review. Nucleus 60:283–297. https://doi.org/10.1007/s13237-017-0214-7 - DOI
    1. Amiteye S (2021) Basic concepts and methodologies of DNA marker systems in plant molecular breeding. Heliyon 7(10):08093. https://doi.org/10.1016/j.heliyon.2021.e08093 - DOI
    1. Annapurna D, Warrier RR, Arunkumar AN et al (2021) Development, characterization, functional annotation and validation of genomic and genic-SSR markers using de novo next generation sequencing in Melia dubia Cav. 3 Biotech 11:310. https://doi.org/10.1007/s13205-021-02858-w - DOI - PubMed - PMC
    1. Babraham Bioinformatics (2017) FastQC A quality control tool for high throughput sequence data. https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ . Accessed 24 May 2017

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