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. 2022 Sep 28;37(1):59-68.
doi: 10.7555/JBR.36.20220094. Online ahead of print.

Peripheral CD4 +CD8 + double positive T cells: A potential marker to evaluate renal impairment susceptibility during systemic lupus erythematosus

Kai Chang  1 , Wanlin Na  1 , Chenxia Liu  2 , Hongxuan Xu  3 , Yuan Liu  1 , Yanyan Wang  1 , Zhongyong Jiang  1   4
Affiliations

Peripheral CD4 +CD8 + double positive T cells: A potential marker to evaluate renal impairment susceptibility during systemic lupus erythematosus

Kai Chang et al. J Biomed Res. .

Abstract

Lupus nephritis (LN) has a high incidence in systemic lupus erythematosus (SLE) patients, but there is a lack of sensitive predictive markers. The purpose of the study was to investigate the association between the CD4 +CD8 + double positive T (DPT) lymphocytes and LN. The study included patients with SLE without renal impairment (SLE-NRI), LN, nephritic syndrome (NS), or nephritis. Peripheral blood lymphocyte subsets were analyzed by flow cytometry. Biochemical measurements were performed with peripheral blood in accordance with the recommendations proposed by the National Center for Clinical Laboratories. The proportions of DPT cells in the LN group were significantly higher than that in the SLE-NRI group ( t=4.012, P<0.001), NS group ( t=3.240, P=0.001), and nephritis group ( t=2.57, P=0.011). In the LN group, the risk of renal impairment increased significantly in a DPT cells proportion-dependent manner. The risk of LN was 5.136 times (95% confidence interval, 2.115-12.473) higher in cases with a high proportion of DPT cells than those whose proportion of DPT cells within the normal range. These findings indicated that the proportion of DPT cells could be a potential marker to evaluate LN susceptibility, and the interference of NS and nephritis could be effectively excluded when assessing the risk of renal impairment during SLE with DPT cell proportion.

Keywords: CD4+CD8+ double positive T cells; lupus nephritis; susceptibility; systemic lupus erythematosus.

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Conflict of interest statement

The authors reported no conflict of interests.

Figures

Figure 1
Figure 1
The maps of target lymphocytes and DPT cells by flow cytometry.
Figure 2
Figure 2
Flow cytometry maps of DPT cells with different fluorescence intensities.
Figure 3
Figure 3
The trend chart of MA/UCr, A/G, CD4+CD8+ DPT cells, and T-Cho among the LN, NS, and nephritis groups.
Figure 4
Figure 4
Comparative analysis and distribution map of CD4+CD8+ DPT in four groups.
See this image and copyright information in PMC

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Grants and funding

This work was supported by the Natural Science Foundation of Sichuan Province (Grant No. 2022NSFSC1415), the Special Project of Sichuan Province Traditional Chinese Medicine Administration (Grant No. 2020JC0124), the Management Project of General Hospital of Western Theater Command (Grants No. 2021-XZYG-C22 and 2021-XZYG-C21), and the Spark Young Innovative Talent Project of General Hospital of Western Theater Command.