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Comparative Study
. 1987 Mar;6(3):667-73.
doi: 10.1002/j.1460-2075.1987.tb04806.x.

Xenopus cytoskeletal actin and human c-fos gene promoters share a conserved protein-binding site

T Mohun, N Garrett, R Treisman
Comparative Study

Xenopus cytoskeletal actin and human c-fos gene promoters share a conserved protein-binding site

T Mohun et al. EMBO J. 1987 Mar.

Abstract

Xenopus laevis cytoskeletal actin gene promoters contain a 20-bp sequence homologous to the serum response element (SRE) required for transient human c-fos gene transcription in response to serum factors. Both sequences bind the same factor in HeLa cell extracts, as shown by binding competition, DNase I and dimethylsulphate (DMS) protection and DMS interference assays. A similar protein is present in Xenopus laevis oocytes. Sequences containing the SRE homology are essential for constitutive activity of the actin promoter in both Xenopus and mouse cells, and a synthetic SRE functions as a promoter element in these cells. In mouse cells, transcription of both transfected Xenopus actin and actin/c-fos fusion genes is activated following serum stimulation. These data suggest that the SRE and its cognate protein form part of a regulatory pathway that has been highly conserved during evolution.

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