The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments
- PMID: 3521892
- DOI: 10.1016/0092-8674(86)90741-5
The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments
Abstract
To map the structural features responsible for the 5-fold difference in stability of the E. coli ompA and bla gene transcripts, we have constructed gene fusions that encode chimeric ompA/bla transcripts and a deletion that eliminates a large internal segment of bla mRNA. Shortening of bla transcripts by internal deletion or replacement of the 3' end with the corresponding segment of the ompA transcript had little effect on bla mRNA stability. However, fusion of a 5'-terminal 147 nucleotide segment of the ompA message 5' to full-length or truncated bla transcripts increased the half-life of the bla segments 3- to 5-fold. These and other findings indicate that E. coli transcripts contain discrete structural determinants of stability and instability that can influence the decay rate of linked mRNA segments derived from other genes.
Similar articles
-
The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency.J Bacteriol. 1990 Aug;172(8):4472-81. doi: 10.1128/jb.172.8.4472-4481.1990. J Bacteriol. 1990. PMID: 1695894 Free PMC article.
-
The differential stability of the Escherichia coli ompA and bla mRNA at various growth rates is not correlated to the efficiency of translation.Gene. 1988 Dec 10;72(1-2):141-9. doi: 10.1016/0378-1119(88)90136-9. Gene. 1988. PMID: 3072245
-
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.Proc Natl Acad Sci U S A. 1983 Feb;80(3):653-7. doi: 10.1073/pnas.80.3.653. Proc Natl Acad Sci U S A. 1983. PMID: 6187001 Free PMC article.
-
Isolation of a mRNA instability sequence that is cis-dominant to the ompA stability determinant in Escherichia coli.Gene. 1996 Nov 14;179(2):263-70. doi: 10.1016/s0378-1119(96)00377-0. Gene. 1996. PMID: 8972910
-
Effect of premature termination of translation on mRNA stability depends on the site of ribosome release.Proc Natl Acad Sci U S A. 1987 Jul;84(14):4890-4. doi: 10.1073/pnas.84.14.4890. Proc Natl Acad Sci U S A. 1987. PMID: 2440033 Free PMC article.
Cited by
-
Preferential nuclear compartmentalization of endogenous mink cell focus-forming-related retroviral transcripts.J Exp Med. 1990 May 1;171(5):1443-52. doi: 10.1084/jem.171.5.1443. J Exp Med. 1990. PMID: 2159049 Free PMC article.
-
Transcriptional unit of the murine Thy-1 gene: different distribution of transcription initiation sites in brain.Mol Cell Biol. 1988 Sep;8(9):3847-56. doi: 10.1128/mcb.8.9.3847-3856.1988. Mol Cell Biol. 1988. PMID: 2906111 Free PMC article.
-
In vivo selected promoter and ribosome binding site up-mutations: demonstration that the Escherichia coli bla promoter and a Shine-Dalgarno region with low complementarity to the 16 S ribosomal RNA function in Bacillus subtilis.Mol Gen Genet. 1989 Oct;219(1-2):129-36. doi: 10.1007/BF00261168. Mol Gen Genet. 1989. PMID: 2515427
-
Global mRNA decay and 23S rRNA fragmentation in Gluconobacter oxydans 621H.BMC Genomics. 2018 Oct 16;19(1):753. doi: 10.1186/s12864-018-5111-1. BMC Genomics. 2018. PMID: 30326828 Free PMC article.
-
Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability.Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14118-23. doi: 10.1073/pnas.95.24.14118. Proc Natl Acad Sci U S A. 1998. PMID: 9826663 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
