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. 1987 Mar 1;165(3):914-9.
doi: 10.1084/jem.165.3.914.

Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor

J Van Damme, G Opdenakker, R J Simpson, M R Rubira, S Cayphas, A Vink, A Billiau, J Van Snick

Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor

J Van Damme et al. J Exp Med. .

Abstract

A factor that promotes the growth of certain B cell hybridomas and of plasmacytomas is shown to be produced by normal human fibroblasts and by a line of human osteosarcoma cells (MG-63) after treatment with IL-1 or TNF. The hybridoma-plasmacytoma growth factor (HPGF) is identified with a 26 kD protein whose mRNA was previously shown to be induced in the same cells by the same inducers. First, poly(A)-rich RNA extracted from IL-1-treated cells could be enriched in HPGF-mRNA content by hybridization to 26 kD cDNA. Second, MG-63-derived HPGF purified to electrophoretic homogeneity was subjected to amino acid sequence analysis, whereby the NH2-terminal sequence was found to match the nucleotide sequence of a 26 kD cDNA clone.

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References

    1. J Gen Virol. 1985 Apr;66 ( Pt 4):693-700 - PubMed
    1. Nature. 1985 Mar 21-27;314(6008):266-8 - PubMed
    1. Eur J Biochem. 1985 Oct 15;152(2):253-7 - PubMed
    1. Cell. 1986 Jun 6;45(5):659-66 - PubMed
    1. J Interferon Res. 1986 Apr;6(2):161-70 - PubMed

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