Amplification of HTLV-III/LAV infection by antigen-induced activation of T cells and direct suppression by virus of lymphocyte blastogenic responses
- PMID: 3493285
Amplification of HTLV-III/LAV infection by antigen-induced activation of T cells and direct suppression by virus of lymphocyte blastogenic responses
Abstract
Peripheral blood mononuclear cells (PBMNC) from healthy donors immune to the soluble antigens tetanus toxoid (TT) and/or keyhole limpet hemocyanin (KLH) were exposed to infectious human T lymphotropic virus, type III/lymphadenopathy-associated virus (HTLV-III/LAV) with and without prior activation by TT or KLH. After exposure to the virus, PBMNC that had been activated by antigen were 10 to 100 times more susceptible to viral replication, as estimated by measurement of production of reverse transcriptase and viral antigens, than PBMNC that had been preincubated without antigen. In addition, exposure of PBMNC to HTLV-III/LAV led to a loss of lymphocyte blastogenic responses after 2 to 3 wk in culture. HTLV-III/LAV-induced inhibition of lymphocyte blastogenic responses occurred in the absence of detectable production of RT but required the use of live rather than heat-inactivated virus. These results demonstrate that HTLV-III/LAV infection is amplified by antigen-induced activation of PBMNC, and that low levels of HTLV-III/LAV infection in vitro can suppress lymphocyte blastogenic responses. This study provides an in vitro model for the analysis of HTLV-III/LAV-induced immune defects.
Similar articles
-
Cellular tropism of the human retrovirus HTLV-III/LAV. I. Role of T cell activation and expression of the T4 antigen.J Immunol. 1985 Nov;135(5):3151-62. J Immunol. 1985. PMID: 2995487
-
Analysis of effector mechanisms against HTLV-I- and HTLV-III/LAV-infected lymphoid cells.J Immunol. 1986 May 15;136(10):3619-24. J Immunol. 1986. PMID: 2422259
-
Mechanisms of B cell activation in patients with acquired immunodeficiency syndrome and related disorders. Contribution of antibody-producing B cells, of Epstein-Barr virus-infected B cells, and of immunoglobulin production induced by human T cell lymphotropic virus, type III/lymphadenopathy-associated virus.J Clin Invest. 1986 Aug;78(2):439-47. doi: 10.1172/JCI112595. J Clin Invest. 1986. PMID: 3016028 Free PMC article.
-
Immunopathogenesis of the acquired immunodeficiency syndrome.Ann Intern Med. 1985 Nov;103(5):704-9. doi: 10.7326/0003-4819-103-5-704. Ann Intern Med. 1985. PMID: 2996403 Review.
-
Molecular and cellular interactions of HIV-1/HTLV coinfection and impact on AIDS progression.AIDS Rev. 2007 Jul-Sep;9(3):140-9. AIDS Rev. 2007. PMID: 17982939 Review.
Cited by
-
Molecular Understanding of HIV-1 Latency.Adv Virol. 2012;2012:574967. doi: 10.1155/2012/574967. Epub 2012 Apr 4. Adv Virol. 2012. PMID: 22548060 Free PMC article.
-
AIDS-dementia-complex: pathology, pathogenesis and future directions.Ital J Neurol Sci. 1989 Jun;10(3):277-87. doi: 10.1007/BF02333773. Ital J Neurol Sci. 1989. PMID: 2670816 Review.
-
The pathogenetic basis of viral tropism.Am J Pathol. 1989 Sep;135(3):447-55. Am J Pathol. 1989. PMID: 2675622 Free PMC article. Review. No abstract available.
-
Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells.J Clin Microbiol. 1988 Nov;26(11):2371-6. doi: 10.1128/jcm.26.11.2371-2376.1988. J Clin Microbiol. 1988. PMID: 3266220 Free PMC article.
-
Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14031-6. doi: 10.1073/pnas.96.24.14031. Proc Natl Acad Sci U S A. 1999. PMID: 10570193 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources
Medical