Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Sep;84(17):6030-4.
doi: 10.1073/pnas.84.17.6030.

The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation

V Kruys, M Wathelet, P Poupart, R Contreras, W Fiers, J Content, G Huez

The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation

V Kruys et al. Proc Natl Acad Sci U S A. 1987 Sep.

Abstract

In vitro-transcribed human interferon-beta (IFN-beta) mRNA, which contains all the sequence of the natural molecule, is poorly translated in a reticulocyte lysate or when injected in Xenopus oocytes. This low level of translation is due to an inhibition by the 5' and 3' untranslated regions (UTRs). Indeed, the replacement of these sequences by those of Xenopus beta-globin mRNA dramatically increases the translational efficiency of the mRNA, especially in oocytes. This phenomenon is not due to a difference in mRNA stability since both native and chimeric mRNAs remain undegraded, at least during the translation period considered. Construction of different chimeric molecules having various combinations of 5' and 3' UTRs from IFN-beta or Xenopus beta-globin mRNA or a small sequence of SP6 polylinker as 5' UTR has revealed that the 3' UTR of IFN-beta in itself has a pronounced inhibitory effect on translation in the two translation systems from animal cells. Indeed, the addition of this 3' UTR at the 3' end of the coding region of a chicken lysozyme mRNA also causes a large decrease of its translational capacity in both systems. However, the nature of the 5' noncoding sequence influences the degree of translation inhibition exerted by the 3' UTR. Remarkably, we observed no difference in translation level when the different mRNAs were tested in a wheat germ extract.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nucleic Acids Res. 1974 Nov;1(11):1385-97 - PubMed
    1. Nature. 1971 Sep 17;233(5316):177-82 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3065-7 - PubMed
    1. Eur J Biochem. 1976 AUG 1;67(1):247-56 - PubMed
    1. Nature. 1980 Jun 19;285(5766):542-7 - PubMed

Publication types

Associated data

LinkOut - more resources