Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jul;7(7):2425-34.
doi: 10.1128/mcb.7.7.2425-2434.1987.

Determinants of rat albumin promoter tissue specificity analyzed by an improved transient expression system

Determinants of rat albumin promoter tissue specificity analyzed by an improved transient expression system

J M Heard et al. Mol Cell Biol. 1987 Jul.

Abstract

The 150-base-pairs region located upstream of the transcriptional start site of the rat albumin gene contains all of the critical sequences necessary for this gene's tissue-specific expression in rat hepatoma cells. In transient expression assays using an improved CAT system or direct mRNA analysis we were able to detect a faithful transcription from the albumin promoter in albumin-negative dedifferentiated H5 hepatoma cells which was 250-fold weaker than in differentiated H4II hepatoma cells producing albumin. This strong tissue specificity could be completely overcome through the cis action of a non-tissue-specific enhancer. Two upstream regions from nucleotides -151 to -119 and from -118 to -94, were required for efficient transcription in H4II cells. Each region contained a sequence motif highly conserved among different species. The effect of the -151/-119 region was strictly tissue specific, while the -118/-94 region was also involved in the low level of transcription observed in H5 cells. Finally, sequences between the CCAAT box and the TATA box also contributed to the overall tissue specificity of rat albumin gene transcription.

PubMed Disclaimer

Similar articles

Cited by

References

    1. EMBO J. 1985 Oct;4(10):2487-91 - PubMed
    1. Mol Cell Biol. 1985 Jun;5(6):1498-511 - PubMed
    1. EMBO J. 1985 Nov;4(11):2831-8 - PubMed
    1. EMBO J. 1985 Sep;4(9):2201-7 - PubMed
    1. Cell. 1986 Jan 31;44(2):261-72 - PubMed

Publication types

Associated data

LinkOut - more resources