In vitro tumoricidal activity of resting and glucan-activated Kupffer cells
- PMID: 3474334
- DOI: 10.1002/jlb.42.1.69
In vitro tumoricidal activity of resting and glucan-activated Kupffer cells
Abstract
Kupffer cells compose 80-90% of fixed tissue macrophages and have been suggested to play an important role in hepatic antitumor resistance. In the present study, the ability of resting and activated Kupffer cells to lyse syngeneic mammary adenocarcinoma BW10232 cells was evaluated. Activated Kupffer cells were isolated from C57Bl/6J mice following single of multiple intravenous (IV) injections of glucan (0.45 mg/mouse), a potent macrophage-activating agent. Mice receiving 5% (w/v) dextrose served as control. Resting Kupffer cells induced significant (P less than .05) 4% and 12% specific lysis of adenocarcinoma cells at target:effector ratios of 1:10 and 1:50, respectively. Kupffer-cell-mediated tumoricidal activity was depressed on day 1 following a single IV injection of glucan. By day 3 postglucan, the antitumor activity of Kupffer cells returned to control levels and was enhanced on days 5 and 10. Following multiple IV injections of glucan on days -5, -3, and -1, Kupffer-cell-mediated cytotoxicity was elevated on days 1 and 4. These observations demonstrate that resting Kupffer cells are significantly cytotoxic to adenocarcinoma cells at T:E ratios of 1:10 and 1:50 and following a transient inhibition of Kupffer-cell-mediated tumoricidal activity, glucan was effective in significantly enhancing the antitumor activity of Kupffer cells.
Similar articles
-
Kupffer cells and liver metastasis. Optimization and limitation of activation of tumoricidal activity.Cancer Metastasis Rev. 1989 Dec;8(3):231-52. doi: 10.1007/BF00047339. Cancer Metastasis Rev. 1989. PMID: 2697472 Review.
-
Comparison of the in vitro cytolytic effect of hepatic, splenic and peritoneal macrophages from glucan-treated mice on sarcoma M5076.Methods Find Exp Clin Pharmacol. 1986 Mar;8(3):157-61. Methods Find Exp Clin Pharmacol. 1986. PMID: 3713379
-
Hemorrhage induces enhanced Kupffer cell cytotoxicity while decreasing peritoneal or splenic macrophage capacity. Involvement of cell-associated tumor necrosis factor and reactive nitrogen.J Immunol. 1991 Dec 15;147(12):4147-54. J Immunol. 1991. PMID: 1753090
-
Beta-glucan functions as an adjuvant for monoclonal antibody immunotherapy by recruiting tumoricidal granulocytes as killer cells.Cancer Res. 2003 Dec 15;63(24):9023-31. Cancer Res. 2003. PMID: 14695221
-
The role of bacterial translocation on Kupffer cell immune function following hemorrhage.Prog Clin Biol Res. 1995;392:209-18. Prog Clin Biol Res. 1995. PMID: 8524926 Review.
Cited by
-
Kupffer cells and liver metastasis. Optimization and limitation of activation of tumoricidal activity.Cancer Metastasis Rev. 1989 Dec;8(3):231-52. doi: 10.1007/BF00047339. Cancer Metastasis Rev. 1989. PMID: 2697472 Review.
-
Natural cytotoxicity of rat hepatic natural killer cells and macrophages against a syngeneic colon adenocarcinoma.Cancer Immunol Immunother. 1988;27(2):137-41. doi: 10.1007/BF00200018. Cancer Immunol Immunother. 1988. PMID: 3416324 Free PMC article.
-
Antimicrobial properties of Kupffer cells.Infect Immun. 1988 Jun;56(6):1430-5. doi: 10.1128/iai.56.6.1430-1435.1988. Infect Immun. 1988. PMID: 3131244 Free PMC article.
-
Efficacy of Pleuran (β-Glucan from Pleurotus ostreatus) in the Management of Herpes Simplex Virus Type 1 Infection.Evid Based Complement Alternat Med. 2020 Apr 13;2020:8562309. doi: 10.1155/2020/8562309. eCollection 2020. Evid Based Complement Alternat Med. 2020. PMID: 32419831 Free PMC article.
-
Enhancement of rat hepatic macrophages by treatment with interleukin-2 and streptococcal preparation OK432, with reference to antitumor activity, soluble factor production and Ia expression.Cancer Immunol Immunother. 1992;35(2):75-82. doi: 10.1007/BF01741853. Cancer Immunol Immunother. 1992. PMID: 1317753 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
