Physical analysis of transcription preinitiation complex assembly on a class II gene promoter
- PMID: 3413495
- DOI: 10.1126/science.3413495
Physical analysis of transcription preinitiation complex assembly on a class II gene promoter
Abstract
Transcription of protein-encoding genes by human RNA polymerase II requires multiple ancillary proteins (transcription factors). Interactions between these proteins and the promoter DNA of a viral class II gene (the major late transcription unit of adenovirus) were investigated by enzymatic and chemical footprinting. The experiments indicated that the assembly of functionally active RNA polymerase II-containing transcription preinitiation complexes requires a complete set of transcription factors, and that both specific protein-DNA and protein-protein interactions are involved. This allows individual steps along the transcription reaction pathway to be tested directly, thus providing a basis for understanding basic transcription initiation mechanisms as well as the regulatory processes that act on them.
Similar articles
-
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.Cell. 1985 Dec;43(2 Pt 1):439-48. doi: 10.1016/0092-8674(85)90174-6. Cell. 1985. PMID: 4075400
-
Kinetic and mechanistic analysis of the RNA polymerase II transcrption reaction at the human interleukin-2 promoter.J Mol Biol. 2001 Dec 14;314(5):993-1006. doi: 10.1006/jmbi.2000.5215. J Mol Biol. 2001. PMID: 11743717
-
The initiator directs the assembly of a transcription factor IID-dependent transcription complex.Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8052-6. doi: 10.1073/pnas.88.18.8052. Proc Natl Acad Sci U S A. 1991. PMID: 1896450 Free PMC article.
-
HIV trans-activation and transcription control mechanisms.New Biol. 1989 Nov;1(2):127-35. New Biol. 1989. PMID: 2562218 Review.
-
Roles of TFIID in transcriptional initiation by RNA polymerase II.Cell. 1991 Sep 20;66(6):1067-70. doi: 10.1016/0092-8674(91)90027-v. Cell. 1991. PMID: 1913802 Review. No abstract available.
Cited by
-
High resolution footprinting of EcoRI and distamycin with Rh(phi)2(bpy)3+, a new photofootprinting reagent.Nucleic Acids Res. 1989 Dec 25;17(24):10259-79. doi: 10.1093/nar/17.24.10259. Nucleic Acids Res. 1989. PMID: 2602152 Free PMC article.
-
Purification of a yeast TATA box-binding protein that exhibits human transcription factor IID activity.Proc Natl Acad Sci U S A. 1989 Jul;86(13):4843-7. doi: 10.1073/pnas.86.13.4843. Proc Natl Acad Sci U S A. 1989. PMID: 2662184 Free PMC article.
-
Analysis of Tat transactivation of human immunodeficiency virus transcription in vitro.Gene Expr. 1992;2(4):391-407. Gene Expr. 1992. PMID: 1282057 Free PMC article.
-
The general transcription factor RAP30 binds to RNA polymerase II and prevents it from binding nonspecifically to DNA.Mol Cell Biol. 1992 Jan;12(1):30-7. doi: 10.1128/mcb.12.1.30-37.1992. Mol Cell Biol. 1992. PMID: 1729606 Free PMC article.
-
Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.Mol Cell Biol. 1992 Jan;12(1):413-21. doi: 10.1128/mcb.12.1.413-421.1992. Mol Cell Biol. 1992. PMID: 1729613 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
