doi: 10.1126/science.3413476.
A ligase-mediated gene detection technique
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- PMID: 3413476
- DOI: 10.1126/science.3413476
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A ligase-mediated gene detection technique
Science.
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Abstract
An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
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