Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

doi:10.1007/s00705-021-05026-w

. 2021 May;166(5):1439-1446.

doi: 10.1007/s00705-021-05026-w. Epub 2021 Mar 8.

Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

Selvin Noé Palacios-Rápalo¹, Luis Adrián De Jesús-González¹, José Manuel Reyes-Ruiz¹, Juan Fidel Osuna-Ramos¹, Carlos Noe Farfan-Morales¹, Ana Lorena Gutiérrez-Escolano², Rosa María Del Ángel³

Affiliations

¹ Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico.
² Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico. alonso@cinvestav.mx.
³ Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico. rmangel@cinvestav.mx.

PMID: 33682072
DOI: 10.1007/s00705-021-05026-w

Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

Selvin Noé Palacios-Rápalo et al. Arch Virol. 2021 May.

. 2021 May;166(5):1439-1446.

doi: 10.1007/s00705-021-05026-w. Epub 2021 Mar 8.

Authors

Affiliations

¹ Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico.
² Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico. alonso@cinvestav.mx.
³ Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico. rmangel@cinvestav.mx.

PMID: 33682072
DOI: 10.1007/s00705-021-05026-w

Abstract

Although dengue virus (DENV) replication occurs in the cytoplasm, the nucleus plays an essential role during infection. Both the capsid protein (C) and non-structural protein 5 (NS5) are translocated into the infected cell nucleus to favor viral replication. Previously, our group reported the nuclear localization of the NS3 protein during DENV infection of mosquito cells; however, the nuclear localization of the DENV NS3 protein in human host cells has not been described. Here, we demonstrated that NS3 is present in the nucleus of Huh7 cells at early infection times, and later, it is mainly located in the cytoplasm.

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References

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1. Lopez-Denman AJ, Mackenzie JM (2017) The IMPORTance of the nucleus during flavivirus replication. Viruses. https://doi.org/10.3390/v9010014 - DOI - PubMed - PMC

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[1] Guzman MG, Harris E (2015) Dengue. Lancet 385:453–465. https://doi.org/10.1016/S0140-6736(14)60572-9 - DOI - PubMed

[2] Guzman MG, Harris E (2015) Dengue. Lancet 385:453–465. https://doi.org/10.1016/S0140-6736(14)60572-9 - DOI - PubMed

[3] Hasan S, Jamdar SF, Alalowi M, Al Beaiji SM (2016) Dengue virus: a global human threat: review of literature. J Int Soc Prev Community Dent 6:1–6. https://doi.org/10.4103/2231-0762.175416 - DOI - PubMed - PMC

[4] Hasan S, Jamdar SF, Alalowi M, Al Beaiji SM (2016) Dengue virus: a global human threat: review of literature. J Int Soc Prev Community Dent 6:1–6. https://doi.org/10.4103/2231-0762.175416 - DOI - PubMed - PMC

[5] Velandia ML, Castellanos JE (2011) Virus del dengue: estructura y ciclo viral. Infectio 15:33–43. https://doi.org/10.1016/S0123-9392(11)70074-1 - DOI

[6] Velandia ML, Castellanos JE (2011) Virus del dengue: estructura y ciclo viral. Infectio 15:33–43. https://doi.org/10.1016/S0123-9392(11)70074-1 - DOI

[7] Mackenzie J (2005) Wrapping things up about virus RNA replication. Traffic Cph Den 6:967–977. https://doi.org/10.1111/j.1600-0854.2005.00339.x - DOI

[8] Mackenzie J (2005) Wrapping things up about virus RNA replication. Traffic Cph Den 6:967–977. https://doi.org/10.1111/j.1600-0854.2005.00339.x - DOI

[9] Lopez-Denman AJ, Mackenzie JM (2017) The IMPORTance of the nucleus during flavivirus replication. Viruses. https://doi.org/10.3390/v9010014 - DOI - PubMed - PMC

[10] Lopez-Denman AJ, Mackenzie JM (2017) The IMPORTance of the nucleus during flavivirus replication. Viruses. https://doi.org/10.3390/v9010014 - DOI - PubMed - PMC

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Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

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Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

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