Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression

doi:10.1002/btpr.3132

. 2021 Jul;37(4):e3132.

doi: 10.1002/btpr.3132. Epub 2021 Apr 15.

Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression

Fatemeh Azadmanesh¹, Mehrab Pourmadadi², Javad Zavar Reza¹, Fatemeh Yazdian², Meisam Omidi³, Bibi Fatemeh Haghirosadat¹

Affiliations

¹ Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran.
³ Protein Research Center, Shahid Beheshti University, Tehran, GC, Iran.

PMID: 33527746
DOI: 10.1002/btpr.3132

Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression

Fatemeh Azadmanesh et al. Biotechnol Prog. 2021 Jul.

. 2021 Jul;37(4):e3132.

doi: 10.1002/btpr.3132. Epub 2021 Apr 15.

Authors

Fatemeh Azadmanesh¹, Mehrab Pourmadadi², Javad Zavar Reza¹, Fatemeh Yazdian², Meisam Omidi³, Bibi Fatemeh Haghirosadat¹

Affiliations

¹ Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran.
³ Protein Research Center, Shahid Beheshti University, Tehran, GC, Iran.

PMID: 33527746
DOI: 10.1002/btpr.3132

Abstract

In this study, a highly porous three-dimensional (3D)-printed wound healing core/shell scaffold fabricated using poly-lactic acid (PLA). The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu-CDs), rosmarinic acid, and chitosan hydrogel. Cu-CDs were synthesized using ammonium hydrogen citrate under hydrothermal conditions. Formulation containing 1 mg ml^-1 concentration of Cu-CDs showed an excellent antibacterial activity against gram bacteria. At 0.25 mg ml^-1 of Cu-CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells. in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold. This finding was confirmed by histological analysis and the relative expression of PDGF, TGF-β, and MMP-1 genes. The biocompatible antibacterial CU-CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.

Keywords: copper carbon dots; three-dimensional bioprinting; wound healing.

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References

REFERENCES

1. Huang X, Zhang Y, Zhang X, Xu L, Chen X, Wei S. Influence of radiation crosslinked carboxymethyl-chitosan/gelatin hydrogel on cutaneous wound healing. Mater Sci Eng C. 2013;33(8):4816-4824.
1. Grzybowski J, Janiak MK, Oldak E, et al. New cytokine dressings. II. Stimulation of oxidative burst in leucocytes in vitro and reduction of viable bacteria within an infected wound. Int J Pharm. 1999;184(2):179-187.
1. Guo Sa, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-229.
1. Schneider LA, Korber A, Grabbe S, Dissemond J. Influence of pH on wound-healing: a new perspective for wound-therapy? Arch Dermatol Res. 2007;298(9):413-420.
1. Srinivasan K, Mahadevan R. Characterization of proton production and consumption associated with microbial metabolism. BMC Biotechnol. 2010;10(1):2-10.

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[1] Huang X, Zhang Y, Zhang X, Xu L, Chen X, Wei S. Influence of radiation crosslinked carboxymethyl-chitosan/gelatin hydrogel on cutaneous wound healing. Mater Sci Eng C. 2013;33(8):4816-4824.

[2] Huang X, Zhang Y, Zhang X, Xu L, Chen X, Wei S. Influence of radiation crosslinked carboxymethyl-chitosan/gelatin hydrogel on cutaneous wound healing. Mater Sci Eng C. 2013;33(8):4816-4824.

[3] Grzybowski J, Janiak MK, Oldak E, et al. New cytokine dressings. II. Stimulation of oxidative burst in leucocytes in vitro and reduction of viable bacteria within an infected wound. Int J Pharm. 1999;184(2):179-187.

[4] Grzybowski J, Janiak MK, Oldak E, et al. New cytokine dressings. II. Stimulation of oxidative burst in leucocytes in vitro and reduction of viable bacteria within an infected wound. Int J Pharm. 1999;184(2):179-187.

[5] Guo Sa, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-229.

[6] Guo Sa, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-229.

[7] Schneider LA, Korber A, Grabbe S, Dissemond J. Influence of pH on wound-healing: a new perspective for wound-therapy? Arch Dermatol Res. 2007;298(9):413-420.

[8] Schneider LA, Korber A, Grabbe S, Dissemond J. Influence of pH on wound-healing: a new perspective for wound-therapy? Arch Dermatol Res. 2007;298(9):413-420.

[9] Srinivasan K, Mahadevan R. Characterization of proton production and consumption associated with microbial metabolism. BMC Biotechnol. 2010;10(1):2-10.

[10] Srinivasan K, Mahadevan R. Characterization of proton production and consumption associated with microbial metabolism. BMC Biotechnol. 2010;10(1):2-10.

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Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression

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Synthesis of a novel nanocomposite containing chitosan as a three-dimensional printed wound dressing technique: Emphasis on gene expression

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