Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Oct;75(1):67-73.
doi: 10.1083/jcb.75.1.67.

Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling

M Schachner, E T Hedley-Whyte, D W Hsu, G Schoonmaker, A Bignami

Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling

M Schachner et al. J Cell Biol. 1977 Oct.

Abstract

Glial fibrillary acidic protein was localized at the electron microscope level in the cerebellum of adult mice by indirect immunoperoxidase histology. In confirmation of previous studies at the light microscope level, the antigen was detectable in astrocytes and their processes, but not in neurons or their processes, or in oligodendroglia. Astrocytic processes were stained in white matter, in the granular layet surrounding synaptic glomerular complexes, and in the molecular layer in the form of radially oriented fibers and of sheaths surrounding Purkinje cell dendrites. Astrocytic endfeet impinging on meninges and perivascular membranes were also antigen positive. In astrocytic perikarya and processes, the immunohistochemical reaction product appears both as a diffuse cytoplasmic label and as elongated strands, which by their distribution and frequency could be considered glial filaments.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Exp Med. 1964 Aug 1;120:253-66 - PubMed
    1. J Cell Biol. 1963 Apr;17:208-12 - PubMed
    1. Brain Res. 1976 Oct 29;116(1):150-7 - PubMed
    1. FEBS Lett. 1976 Jul 15;66(2):281-4 - PubMed
    1. Brain Res. 1973 Jul 27;57(2):343-60 - PubMed

Publication types

Substances