Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan 14;12(1):71.
doi: 10.3390/insects12010071.

Permethrin Resistance in Aedes aegypti Affects Aspects of Vectorial Capacity

Tse-Yu Chen  1 , Chelsea T Smartt  1 , Dongyoung Shin  1
Affiliations

Permethrin Resistance in Aedes aegypti Affects Aspects of Vectorial Capacity

Tse-Yu Chen et al. Insects. .

Abstract

Aedes aegypti, as one of the vectors transmitting several arboviruses, is the main target in mosquito control programs. Permethrin is used to control mosquitoes and Aedes aegypti get exposed due to its overuse and are now resistant. The increasing percentage of permethrin resistant Aedes aegypti has become an important issue around the world and the potential influence on vectorial capacity needs to be studied. Here we selected a permethrin resistant (p-s) Aedes aegypti population from a wild Florida population and confirmed the resistance ratio to its parental population. We used allele-specific PCR genotyping of the V1016I and F1534C sites in the sodium channel gene to map mutations responsible for the resistance. Two important factors, survival rate and vector competence, that impact vectorial capacity were checked. Results indicated the p-s population had 20 times more resistance to permethrin based on LD50 compared to the parental population. In the genotyping study, the p-s population had more homozygous mutations in both mutant sites of the sodium channel gene. The p-s adults survived longer and had a higher dissemination rate for dengue virus than the parental population. These results suggest that highly permethrin resistant Aedes aegypti populations might affect the vectorial capacity, moreover, resistance increased the survival time and vector competence, which should be of concern in areas where permethrin is applied.

Keywords: Aedes aegypti; kdr; permethrin resistance; survival time; vector competence; vectorial capacity.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Relative detoxification gene expression between the p-s population and the Key West population. Three replicates of five pooled female mosquitoes were compared between p-s and Key West populations. The bar indicates the mean with standard deviation and the dots represent the expression level in each sample.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Kramer L.D., Ebel G.D. Dynamics of flavivirus infection in mosquitoes. Adv. Virus Res. 2003;60:187–232. doi: 10.1016/s0065-3527(03)60006-0. - DOI - PubMed
    1. Cuervo-Parra J.A., Cortés T.R., Ramirez-Lepe M. Insecticides Resistance. IntechOpen; Rijeka, Croatia: 2016. Mosquito-Borne Diseases, Pesticides Used for Mosquito Control, and Development of Resistance to Insecticides; pp. 111–134.
    1. Vontas J., Kioulos E., Pavlidi N., Morou E., Della Torre A., Ranson H. Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti. Pestic Biochem. Physiol. 2012;104:126–131. doi: 10.1016/j.pestbp.2012.05.008. - DOI
    1. Bayo F.S. Insecticides Mode of Action in Relation to Their Toxicity to Non-Target Organisms. J. Environ. Anal. Toxicol. 2012;2012:1–9.
    1. Chareonviriyaphap T., Bangs M.J., Suwonkerd W., Kongmee M., Corbel V., Ngoen-Klan R. Review of insecticide resistance and behavioral avoidance of vectors of human diseases in Thailand. Parasites Vectors. 2013;6:280. doi: 10.1186/1756-3305-6-280. - DOI - PMC - PubMed

LinkOut - more resources