Production of interferon alpha by dengue virus-infected human monocytes
- PMID: 3339332
- DOI: 10.1099/0022-1317-69-2-445
Production of interferon alpha by dengue virus-infected human monocytes
Abstract
Human monocytes appear to be very important in the pathogenesis of dengue infection. They are thought to be the most active sites of virus replication during dengue infection. We have analysed interferon (IFN) production by dengue virus from peripheral blood mononuclear cells (PBMC). IFN activity was first detected at 12 h after infection of monocytes and reached a maximum level by 48 h. Non-adherent PBMC depleted of monocytes did not produce detectable levels of IFN, and did not contain dengue antigen-positive cells after exposure to dengue virus. The IFN produced was characterized as IFN-alpha by neutralization tests using specific antisera to HuIFN-alpha, HuIFN-beta and HuIFN-gamma, and by radioimmunoassay. The culture fluids of dengue virus-infected monocytes, which contained IFN-alpha, were able to inhibit infection of human monocytes by dengue virus. These results suggest that IFN-alpha produced by dengue virus-infected monocytes may play an important role in controlling primary dengue virus infection.
Similar articles
-
Induction of IFN-alpha in peripheral blood mononuclear cells by HIV-infected monocytes. Restricted antiviral activity of the HIV-induced IFN.J Immunol. 1992 Jan 15;148(2):422-9. J Immunol. 1992. PMID: 1729362
-
Induction of interferon alpha and gamma from human lymphocytes by dengue virus-infected cells.J Gen Virol. 1986 Aug;67 ( Pt 8):1653-61. doi: 10.1099/0022-1317-67-8-1653. J Gen Virol. 1986. PMID: 3090196
-
Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes.J Exp Med. 1987 Oct 1;166(4):999-1010. doi: 10.1084/jem.166.4.999. J Exp Med. 1987. PMID: 3116147 Free PMC article.
-
Human T cell responses to dengue virus antigens. Proliferative responses and interferon gamma production.J Clin Invest. 1989 Feb;83(2):506-13. doi: 10.1172/JCI113911. J Clin Invest. 1989. PMID: 2521489 Free PMC article.
-
The monocyte-macrophage-mast cell axis in dengue pathogenesis.J Biomed Sci. 2018 Nov 8;25(1):77. doi: 10.1186/s12929-018-0482-9. J Biomed Sci. 2018. PMID: 30409217 Free PMC article. Review.
Cited by
-
Characterization of Dendritic Cell-Derived Extracellular Vesicles During Dengue Virus Infection.Front Microbiol. 2018 Aug 6;9:1792. doi: 10.3389/fmicb.2018.01792. eCollection 2018. Front Microbiol. 2018. PMID: 30131785 Free PMC article.
-
Immune responses to dengue virus in the skin.Open Biol. 2018 Aug;8(8):180087. doi: 10.1098/rsob.180087. Open Biol. 2018. PMID: 30135238 Free PMC article. Review.
-
Both viremia and cytokine levels associate with the lack of severe disease in secondary dengue 1 infection among adult Chinese patients.PLoS One. 2010 Dec 29;5(12):e15631. doi: 10.1371/journal.pone.0015631. PLoS One. 2010. PMID: 21206915 Free PMC article.
-
Micronutrients and dengue.Am J Trop Med Hyg. 2014 Nov;91(5):1049-56. doi: 10.4269/ajtmh.14-0142. Epub 2014 Sep 8. Am J Trop Med Hyg. 2014. PMID: 25200269 Free PMC article. Review.
-
Secretion of Galectin-9 as a DAMP during Dengue Virus Infection in THP-1 Cells.Int J Mol Sci. 2017 Jul 28;18(8):1644. doi: 10.3390/ijms18081644. Int J Mol Sci. 2017. PMID: 28788062 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
