Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Apr;23(4):2132-2151.
doi: 10.1111/1462-2920.15382. Epub 2021 Jan 12.

Induction of antibiotic specialized metabolism by co-culturing in a collection of phyllosphere bacteria

Shan Shan Qi  1 , Alexander Bogdanov  2   3 , Margo Cnockaert  1 , Tessa Acar  1   4 , Sarah Ranty-Roby  4 , Tom Coenye  5 , Peter Vandamme  1 , Gabriele M König  2 , Max Crüsemann  2 , Aurélien Carlier  1   4
Affiliations

Induction of antibiotic specialized metabolism by co-culturing in a collection of phyllosphere bacteria

Shan Shan Qi et al. Environ Microbiol. 2021 Apr.

Abstract

A diverse set of bacteria live on the above-ground parts of plants, composing the phyllosphere, and play important roles for plant health. Phyllosphere microbial communities assemble in a predictable manner and diverge from communities colonizing other plant organs or the soil. However, how these communities differ functionally remains obscure. We assembled a collection of 258 bacterial isolates representative of the most abundant taxa of the phyllosphere of Arabidopsis and a shared soil inoculum. We screened the collection for the production of metabolites that inhibit the growth of Gram-positive and Gram-negative bacteria either in isolation or in co-culture. We found that isolates capable of constitutive antibiotic production in monoculture were significantly enriched in the soil fraction. In contrast, the proportion of binary cultures resulting in the production of growth inhibitory compounds differed only marginally between the phyllosphere and soil fractions. This shows that the phyllosphere may be a rich resource for potentially novel molecules with antibiotic activity, but that production or activity is dependent upon induction by external signals or cues. Finally, we describe the isolation of antimicrobial acyloin metabolites from a binary culture of Arabidopsis phyllosphere isolates, which inhibit the growth of clinically relevant Acinetobacter baumannii.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Abrudan, M.I., Smakman, F., Grimbergen, A.J., Westhoff, S., Miller, E.L., Van Wezel, G.P., and Rozen, D.E. (2015) Socially mediated induction and suppression of antibiosis during bacterial coexistence. Proc Natl Acad Sci U S A 112: 11054-11059.
    1. Agler, M.T., Ruhe, J., Kroll, S., Morhenn, C., Kim, S.-T., Weigel, D., and Kemen, E.M. (2016) Microbial hub taxa link host and abiotic factors to plant microbiome variation. PLoS Biol 14: e1002352.
    1. Aguirre-von-Wobeser, E., Eguiarte, L.E., Souza, V., and Soberon-Chavez, G. (2015) Theoretical analysis of the cost of antagonistic activity for aquatic bacteria in oligotrophic environments. Front Microbiol 6: 490.
    1. Alderman, D., and Smith, P. (2001) Development of draft protocols of standard reference methods for antimicrobial agent susceptibility testing of bacteria associated with fish diseases. Aquaculture 196: 211-243.
    1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.

Publication types

Substances

LinkOut - more resources