Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan 12;54(1):176-190.e7.
doi: 10.1016/j.immuni.2020.11.007. Epub 2020 Dec 16.

A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages

Jung-Seok Kim  1 , Masha Kolesnikov  1 , Shany Peled-Hajaj  2 , Isabelle Scheyltjens  3 , Yuan Xia  1 , Sebastien Trzebanski  1 , Zhana Haimon  1 , Anat Shemer  1 , Alisa Lubart  2 , Hannah Van Hove  3 , Louise Chappell-Maor  1 , Sigalit Boura-Halfon  1 , Kiavash Movahedi  3 , Pablo Blinder  4 , Steffen Jung  5
Affiliations
Free article

A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages

Jung-Seok Kim et al. Immunity. .
Free article

Abstract

The developmental and molecular heterogeneity of tissue macrophages is unravelling, as are their diverse contributions to physiology and pathophysiology. Moreover, also given tissues harbor macrophages in discrete anatomic locations. Functional contributions of specific cell populations can in mice be dissected using Cre recombinase-mediated mutagenesis. However, single promoter-based Cre models show limited specificity for cell types. Focusing on macrophages in the brain, we establish here a binary transgenic system involving complementation-competent NCre and CCre fragments whose expression is driven by distinct promoters: Sall1ncre: Cx3cr1ccre mice specifically target parenchymal microglia and compound transgenic Lyve1ncre: Cx3cr1ccre animals target vasculature-associated macrophages, in the brain, as well as other tissues. We imaged the respective cell populations and retrieved their specific translatomes using the RiboTag in order to define them and analyze their differential responses to a challenge. Collectively, we establish the value of binary transgenesis to dissect tissue macrophage compartments and their functions.

Keywords: Lyve1 BAM; RiboTag; binary transgenic; brain macrophages; intersectional genetics; meninges; microglia; perivascular macrophages; pia mater; split cre.

PubMed Disclaimer

Conflict of interest statement

Declaration of Interests The authors declare that they have no competing interests.

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources