Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec;99(12):6745-6752.
doi: 10.1016/j.psj.2020.09.050. Epub 2020 Oct 1.

Dietary chitosan oligosaccharides alleviate heat stress-induced intestinal oxidative stress and inflammatory response in yellow-feather broilers

Ruixia Lan  1 , Yaxuan Li  1 , Qingqing Chang  1 , Zhihui Zhao  2
Affiliations

Dietary chitosan oligosaccharides alleviate heat stress-induced intestinal oxidative stress and inflammatory response in yellow-feather broilers

Ruixia Lan et al. Poult Sci. 2020 Dec.

Abstract

The purpose of this study was to evaluate the effects of chitosan oligosaccharides (COS) on intestinal permeability, morphology, antioxidant status, and inflammatory response in heat-stressed broilers. A total of 108 thirty-five-day-old Chinese yellow-feather broilers (body weight 470.31 ± 13.15 g) were randomly allocated to 3 dietary treatments as follows: CON group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00-18:00 and 24°C for the rest time); HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Each treatment had 6 replication pens and 6 broilers per pen. Compared with the CON group, heat stress decreased (P < 0.05) the relative weight of duodenum and jejunum; the relative length and villus height (VH) of duodenum, jejunum, and ileum; the ileum VH to crypt depth ratio; duodenum mucosal catalase (CAT) activity; and jejunum mucosal glutathione peroxidase (GSH-Px) and CAT activity, whereas it increased (P < 0.05) serum diamine oxidase (DAO) activity and D-lactate acid (D-LA) content, duodenum and jejunum mucosal malondialdehyde (MDA) and interleukin-1β (IL-1β) content, and ileum mucosal tumor necrosis factor-α content. Compared to the HS group, dietary COS supplementation increased (P < 0.05) the relative length of duodenum, jejunum, and ileum; the VH of jejunum and ileum; and duodenum and jejunum mucosal GSH-Px activity, whereas it decreased (P < 0.05) serum DAO activity and D-LA concentration and duodenum and jejunum mucosal MDA and IL-1β content. These results suggested that dietary COS supplementation had beneficial effects on intestinal morphology by increasing jejunum and ileum VH; permeability by decreasing serum DAO activity and D-LA content; antioxidant capacity by decreasing duodenum and jejunum mucosal MDA content and by increasing duodenum and jejunum GSH-Px activity; and inflammatory response by decreasing duodenum and jejunum mucosal IL-1β content.

Keywords: chitosan oligosaccharide; heat stress; intestinal inflammation; intestinal oxidative status; yellow-feather broiler.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effects of chitosan oligosaccharides (COS) on (A) serum diamine oxidase (DAO) and (B) D-lactate acid (D-LA) in yellow-feather heat-stressed broilers. CON group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00–18:00 and 24°C for the rest time); HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Values are mean ± SE (n = 6). The values with different superscript letters are different (P < 0.05).
Figure 2
Figure 2
Effects of chitosan oligosaccharides (COS) on antioxidant status of the small intestine in yellow-feather heat-stressed broilers. CON group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00–18:00 and 24°C for the rest time); HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Abbreviations: CAT, catalase; GSH-Px, glutathione peroxidase; MDA, malondialdehyde; SOD, superoxide dismutase. Values are mean ± SE (n = 6). The values with different superscript letters are different (P < 0.05).
Figure 3
Figure 3
Effects of chitosan oligosaccharide (COS) on inflammatory cytokines of small intestine in yellow-feather heat-stressed broilers. CON group, basal diet and raised under normal temperature (24°C); HS group, basal diet and raised under cycle heat stress (34°C from 10:00–18:00 and 24°C for the rest time); HSC group, basal diet with 200 mg/kg COS supplementation and raised under cycle heat stress. Abbreviations: IL-1β, interleukin-1β; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. Values are mean ± SE (n = 6). The values with different superscript letters are different (P < 0.05).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Akbari P., Braber S., Alizadeh A., Verheijden K.A., Schoterman M.H., Kraneveld A.D., Garssen J., Fink-Gremmels J. Galacto-oligosaccharides protect the intestinal barrier by maintaining the tight junction network and modulating the inflammatory responses after a challenge with the mycotoxin deoxynivalenol in human Caco-2 cell monolayers and B6C3F1 mice. J. Nutr. 2015;145:1604–1613. - PubMed
    1. Brandsma E., Houben T., Fu J., Shiri-Sverdlov R., Hofker M.H. The immunity–diet–microbiota axis in the development of metabolic syndrome. Curr. Opin. Lipidol. 2015;26:73–81. - PubMed
    1. Cheng Y., Chen Y., Chen R., Su Y., Zhang R., He Q., Wang K., Wen C., Zhou Y. Dietary mannan oligosaccharide ameliorates cyclic heat stress-induced damages on intestinal oxidative status and barrier integrity of broilers. Poult. Sci. 2019;98:4767–4776. - PubMed
    1. Cheng Y., Du M., Xu Q., Chen Y., Wen C., Zhou Y. Dietary mannan oligosaccharide improves growth performance, muscle oxidative status, and meat quality in broilers under cyclic heat stress. J. Therm. Biol. 2018;75:106–111. - PubMed
    1. Dann S.M., Spehlmann M.E., Hammond D.C., Iimura M., Hase K., Choi L.J., Hanson E., Eckmann L. IL-6-dependent mucosal protection prevents establishment of a microbial niche for attaching/effacing lesion-forming enteric bacterial pathogens. J. Immunol. 2008;180:6816–6826. - PMC - PubMed