doi: 10.1002/j.1460-2075.1987.tb02652.x.
Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers
Affiliations
- PMID: 3322806
- PMCID: PMC553786
- DOI: 10.1002/j.1460-2075.1987.tb02652.x
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Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers
EMBO J.
1987 Nov.
Abstract
An enhancer binding factor, designated KBF1, has been purified from the nuclear extract of mouse BW5147 thymoma cells by five column chromatography steps including a sequence-specific DNA affinity column. Gel retardation and footprint analysis have shown that purified KBF1 has a binding activity specific for both H-2 and beta 2-microglobulin enhancer sequences. After SDS-polyacrylamide gel electrophoresis of the most purified preparation a 48-kd protein showed, after elution and renaturation, a binding activity to both enhancer sequences. These findings suggest that the expression of both H-2 and beta 2-microglobulin genes utilizes a common regulatory mechanism.
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