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. 1987 Jul;6(7):2117-22.
doi: 10.1002/j.1460-2075.1987.tb02478.x.

Disruption of the outer membrane restores protein import to trypsin-treated yeast mitochondria

M Ohba  1 , G Schatz
Affiliations

Disruption of the outer membrane restores protein import to trypsin-treated yeast mitochondria

M Ohba et al. EMBO J. 1987 Jul.

Abstract

Treatment of isolated yeast mitochondria with high levels (1 mg/ml) of trypsin severely inhibits protein import but does not destroy the integrity of the outer membrane or abolish mitochondrial energy coupling. If the outer membrane of these trypsin-inactivated mitochondria is disrupted by osmotic shock, the resulting mitoplasts are again able to import proteins. Protein import into mitoplasts, like that into intact mitochondria, is energy-dependent; however, whereas import into mitochondria is inhibited by antibody against 45-kd proteins of the outer membrane [Ohba and Schatz, EMBO J., 6, 2109-2115 (1987)], import into mitoplasts not affected by this antibody. Protein import into mitoplasts appears to bypass one or more steps normally occurring at the mitochondrial surface.

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