A yeast activity can substitute for the HeLa cell TATA box factor
- PMID: 3290688
- DOI: 10.1038/334077a0
A yeast activity can substitute for the HeLa cell TATA box factor
Abstract
Most class B (II) promoter regions from higher eukaryotes contain the TATA box and upstream and enhancer elements. Both the upstream and enhancer elements and their cognate factors have regulatory functions, whereas the TATA sequence interacts with the TATA box factor BTF1 to position RNA polymerase B and its ancillary initiation factors (STF, BTF2 and BTF3) to direct the initiation of transcription approximately 30 base pairs downstream. In many respects, class B promoter regions from the unicellular eukaryote Saccharomyces cerevisiae are similarly organized, containing upstream activating sequences that bear many similarities to enhancers. Although they are essential for initiation, the yeast TATA sequences are located at variable distances and further from the start sites (40-120 base pairs), whose locations are primarily determined by an initiator element. The basic molecular mechanisms that control initiation of transcription are known to be conserved from yeast to man: the yeast transcriptional transactivator GAL4 can activate a minimal TATA box-containing promoter in human HeLa cells, and a human inducible enhancer factor, the oestrogen receptor, can activate a similar minimal promoter in yeast. This striking evolutionary conservation prompted us to look for the presence in yeast of an activity that could possibly substitute for the human TATA box factor. We report here the existence of such an activity in yeast extracts.
Similar articles
-
Function of a yeast TATA element-binding protein in a mammalian transcription system.Nature. 1988 Jul 7;334(6177):37-42. doi: 10.1038/334037a0. Nature. 1988. PMID: 3290687
-
A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID.Nature. 1990 Nov 1;348(6296):86-8. doi: 10.1038/348086a0. Nature. 1990. PMID: 2234067
-
Basal components of the transcription apparatus (RNA polymerase II, TATA-binding protein) contain activation domains: is the repetitive C-terminal domain (CTD) of RNA polymerase II a "portable enhancer domain"?Mol Reprod Dev. 1994 Oct;39(2):215-25. doi: 10.1002/mrd.1080390215. Mol Reprod Dev. 1994. PMID: 7826625
-
Transcription elements and factors of RNA polymerase B promoters of higher eukaryotes.CRC Crit Rev Biochem. 1988;23(2):77-120. doi: 10.3109/10409238809088317. CRC Crit Rev Biochem. 1988. PMID: 3048889 Review.
-
Conservation and evolution of transcriptional mechanisms in eukaryotes.Trends Genet. 1992 Jan;8(1):27-32. doi: 10.1016/0168-9525(92)90021-u. Trends Genet. 1992. PMID: 1369732 Review.
Cited by
-
cis- and trans-acting regulatory elements of the yeast URA3 promoter.Mol Cell Biol. 1990 Oct;10(10):5257-70. doi: 10.1128/mcb.10.10.5257-5270.1990. Mol Cell Biol. 1990. PMID: 2204810 Free PMC article.
-
Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.Proc Natl Acad Sci U S A. 1990 Dec;87(23):9158-62. doi: 10.1073/pnas.87.23.9158. Proc Natl Acad Sci U S A. 1990. PMID: 2251257 Free PMC article.
-
Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma.Oncol Lett. 2019 Jan;17(1):789-796. doi: 10.3892/ol.2018.9699. Epub 2018 Nov 14. Oncol Lett. 2019. PMID: 30655831 Free PMC article.
-
Mutations that increase the activity of a transcriptional activator in yeast and mammalian cells.Proc Natl Acad Sci U S A. 1990 Mar;87(6):2127-31. doi: 10.1073/pnas.87.6.2127. Proc Natl Acad Sci U S A. 1990. PMID: 2179950 Free PMC article.
-
Core promoter of the mouse myelin basic protein gene governs brain-specific transcription in vitro.EMBO J. 1990 Oct;9(10):3101-8. doi: 10.1002/j.1460-2075.1990.tb07507.x. EMBO J. 1990. PMID: 1698607 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
