The protein factor which binds to the upstream activating sequence of Saccharomyces cerevisiae ENO1 gene
- PMID: 3279392
- PMCID: PMC336324
- DOI: 10.1093/nar/16.4.1407
The protein factor which binds to the upstream activating sequence of Saccharomyces cerevisiae ENO1 gene
Abstract
Using a gel retardation assay it was shown that the 87 bp DNA fragment (UAS87) containing the upstream activating sequence (UAS) of S. cerevisiae EN01 gene and a nuclear extract gave rise to three migration-retarded species specific to UAS87. Heat- or proteinase-treatment of the nuclear extract revealed that these species were protein-DNA complexes. The precise binding region of the protein identified by DNaseI protection analysis was found to include a CCAAACA sequence which forms a dyad-symmetrical structure. The amount of one of the three migration-retarded species significantly increased when cells were grown in medium containing a gluconeogenic carbon source. The introduction of pGCR8, a multicopy plasmid containing GCR1 gene, a regulatory gene controlling the expression of several glycolytic enzymes, showed no effect on the amount of three migration-retarded species.
Similar articles
-
Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.Mol Cell Biol. 1990 Sep;10(9):4872-85. doi: 10.1128/mcb.10.9.4872-4885.1990. Mol Cell Biol. 1990. PMID: 2201905 Free PMC article.
-
A positive regulatory sequence of the Saccharomyces cerevisiae ENO1 gene.J Biochem. 1987 Jul;102(1):181-9. doi: 10.1093/oxfordjournals.jbchem.a122031. J Biochem. 1987. PMID: 3117780
-
Purification and characterization of a nuclear factor which binds specifically to the upstream activation sequence of Saccharomyces cerevisiae enolase 1 gene.Eur J Biochem. 1989 Sep 15;184(2):305-11. doi: 10.1111/j.1432-1033.1989.tb15020.x. Eur J Biochem. 1989. PMID: 2676532
-
Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences.Mol Cell Biol. 1987 Aug;7(8):2753-61. doi: 10.1128/mcb.7.8.2753-2761.1987. Mol Cell Biol. 1987. PMID: 3313003 Free PMC article.
-
Sequences within an upstream activation site in the yeast enolase gene ENO2 modulate repression of ENO2 expression in strains carrying a null mutation in the positive regulatory gene GCR1.Mol Cell Biol. 1990 Sep;10(9):4863-71. doi: 10.1128/mcb.10.9.4863-4871.1990. Mol Cell Biol. 1990. PMID: 2201904 Free PMC article.
Cited by
-
The UAS of the yeast PGK gene is composed of multiple functional elements.Nucleic Acids Res. 1988 Sep 12;16(17):8245-60. doi: 10.1093/nar/16.17.8245. Nucleic Acids Res. 1988. PMID: 3047680 Free PMC article.
-
A REB1-binding site is required for GCN4-independent ILV1 basal level transcription and can be functionally replaced by an ABF1-binding site.Mol Cell Biol. 1992 Dec;12(12):5516-26. doi: 10.1128/mcb.12.12.5516-5526.1992. Mol Cell Biol. 1992. PMID: 1448083 Free PMC article.
-
Mutations in GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, suppress the temperature-sensitive growth of hpr1 mutants.Genetics. 1996 Apr;142(4):1095-103. doi: 10.1093/genetics/142.4.1095. Genetics. 1996. PMID: 8846890 Free PMC article.
-
Elements involved in S-adenosylmethionine-mediated regulation of the Saccharomyces cerevisiae MET25 gene.Mol Cell Biol. 1989 Aug;9(8):3292-8. doi: 10.1128/mcb.9.8.3292-3298.1989. Mol Cell Biol. 1989. PMID: 2552290 Free PMC article.
-
Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.Mol Cell Biol. 1990 Sep;10(9):4872-85. doi: 10.1128/mcb.10.9.4872-4885.1990. Mol Cell Biol. 1990. PMID: 2201905 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
Miscellaneous