Single-cell omics in ageing: a young and growing field

doi:10.1038/s42255-020-0196-7

Review

. 2020 Apr;2(4):293-302.

doi: 10.1038/s42255-020-0196-7. Epub 2020 Apr 20.

Single-cell omics in ageing: a young and growing field

Xiaojuan He^{1

2}, Sebastian Memczak³, Jing Qu^{4

5

6}, Juan Carlos Izpisua Belmonte⁷, Guang-Hui Liu^{8

9

10

11}

Affiliations

¹ Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing, China.
² State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
³ Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
⁴ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. qujing@ioz.ac.cn.
⁵ University of Chinese Academy of Sciences, Beijing, China. qujing@ioz.ac.cn.
⁶ Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing, China. qujing@ioz.ac.cn.
⁷ Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA. belmonte@salk.edu.
⁸ Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing, China. ghliu@ioz.ac.cn.
⁹ State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. ghliu@ioz.ac.cn.
¹⁰ University of Chinese Academy of Sciences, Beijing, China. ghliu@ioz.ac.cn.
¹¹ Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing, China. ghliu@ioz.ac.cn.

PMID: 32694606
DOI: 10.1038/s42255-020-0196-7

Review

Single-cell omics in ageing: a young and growing field

Xiaojuan He et al. Nat Metab. 2020 Apr.

. 2020 Apr;2(4):293-302.

doi: 10.1038/s42255-020-0196-7. Epub 2020 Apr 20.

Authors

Xiaojuan He^{1

2}, Sebastian Memczak³, Jing Qu^{4

5

6}, Juan Carlos Izpisua Belmonte⁷, Guang-Hui Liu^{8

9

10

11}

Affiliations

¹ Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing, China.
² State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
³ Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
⁴ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. qujing@ioz.ac.cn.
⁵ University of Chinese Academy of Sciences, Beijing, China. qujing@ioz.ac.cn.
⁶ Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing, China. qujing@ioz.ac.cn.
⁷ Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA. belmonte@salk.edu.
⁸ Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing, China. ghliu@ioz.ac.cn.
⁹ State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. ghliu@ioz.ac.cn.
¹⁰ University of Chinese Academy of Sciences, Beijing, China. ghliu@ioz.ac.cn.
¹¹ Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing, China. ghliu@ioz.ac.cn.

PMID: 32694606
DOI: 10.1038/s42255-020-0196-7

Abstract

Organismal ageing results from interlinked molecular changes in multiple organs over time. The study of ageing at the molecular level is complicated by varying decay characteristics and kinetics-both between and within organs-driven by intrinsic and extracellular factors. Emerging single-cell omics methods allow for molecular and spatial profiling of cells, and probing of regulatory states and cell-fate determination, thus providing promising tools for unravelling the heterogeneous process of ageing and making it amenable to intervention. These new strategies are enabled by advances in genomic, epigenomic and transcriptomic technologies. Combined with methods for proteome and metabolome analysis, single-cell techniques provide multidimensional, integrated data with unprecedented detail and throughput. Here, we provide an overview of the current state, and perspectives on the future, of this emerging field. We discuss how single-cell approaches can advance understanding of mechanisms underlying organismal ageing and aid in the development of interventions for ageing and ageing-associated diseases.

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References

1. Stuart, T. & Satija, R. Integrative single-cell analysis. Nat. Rev. Genet. 20, 257–272 (2019). - PubMed - DOI
1. Prakadan, S. M., Shalek, A. K. & Weitz, D. A. Scaling by shrinking: empowering single-cell ‘omics’ with microfluidic devices. Nat. Rev. Genet. 18, 345–361 (2017). - PubMed - PMC - DOI
1. Gawad, C., Koh, W. & Quake, S. R. Single-cell genome sequencing: current state of the science. Nat. Rev. Genet. 17, 175–188 (2016). - PubMed - DOI
1. Schwartzman, O. & Tanay, A. Single-cell epigenomics: techniques and emerging applications. Nat. Rev. Genet. 16, 716–726 (2015). - PubMed - DOI
1. Wu, A. R., Wang, J., Streets, A. M. & Huang, Y. Single-cell transcriptional analysis. Annu. Rev. Anal. Chem. (Palo Alto Calif) 10, 439–462 (2017). - DOI

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[1] Stuart, T. & Satija, R. Integrative single-cell analysis. Nat. Rev. Genet. 20, 257–272 (2019). - PubMed - DOI

[2] Stuart, T. & Satija, R. Integrative single-cell analysis. Nat. Rev. Genet. 20, 257–272 (2019). - PubMed - DOI

[3] Prakadan, S. M., Shalek, A. K. & Weitz, D. A. Scaling by shrinking: empowering single-cell ‘omics’ with microfluidic devices. Nat. Rev. Genet. 18, 345–361 (2017). - PubMed - PMC - DOI

[4] Prakadan, S. M., Shalek, A. K. & Weitz, D. A. Scaling by shrinking: empowering single-cell ‘omics’ with microfluidic devices. Nat. Rev. Genet. 18, 345–361 (2017). - PubMed - PMC - DOI

[5] Gawad, C., Koh, W. & Quake, S. R. Single-cell genome sequencing: current state of the science. Nat. Rev. Genet. 17, 175–188 (2016). - PubMed - DOI

[6] Gawad, C., Koh, W. & Quake, S. R. Single-cell genome sequencing: current state of the science. Nat. Rev. Genet. 17, 175–188 (2016). - PubMed - DOI

[7] Schwartzman, O. & Tanay, A. Single-cell epigenomics: techniques and emerging applications. Nat. Rev. Genet. 16, 716–726 (2015). - PubMed - DOI

[8] Schwartzman, O. & Tanay, A. Single-cell epigenomics: techniques and emerging applications. Nat. Rev. Genet. 16, 716–726 (2015). - PubMed - DOI

[9] Wu, A. R., Wang, J., Streets, A. M. & Huang, Y. Single-cell transcriptional analysis. Annu. Rev. Anal. Chem. (Palo Alto Calif) 10, 439–462 (2017). - DOI

[10] Wu, A. R., Wang, J., Streets, A. M. & Huang, Y. Single-cell transcriptional analysis. Annu. Rev. Anal. Chem. (Palo Alto Calif) 10, 439–462 (2017). - DOI

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Single-cell omics in ageing: a young and growing field

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Single-cell omics in ageing: a young and growing field

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