doi: 10.1007/BF02616174.
Cell size in aging monolayer cultures
- PMID: 326658
- DOI: 10.1007/BF02616174
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Cell size in aging monolayer cultures
In Vitro.
1977 May.
Abstract
Changes in the size of the area covered by individual cultured WI-38 cells as the cultures age have been studied by using a new microphotographic paper cutout technique. This method is nondestructive and noninstrusive and avoids a number of artifacts which can occur in the measurement of suspended cells. The measurements reveal that the decreased cell yield of late passage cultures reflects not only the appearance of a subpopulation of larger cells but also the failure of the cells to utilize all the growth surface available to them.
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