Immune enhancement of pulmonary clearance of nontypable Haemophilus influenzae
- PMID: 3257203
- PMCID: PMC259254
- DOI: 10.1128/iai.56.1.182-190.1988
Immune enhancement of pulmonary clearance of nontypable Haemophilus influenzae
Abstract
BALB/c mice systemically immunized by intraperitoneal injection with whole, viable cells of two different strains of nontypable Haemophilus influenzae (NTHI) exhibited a markedly enhanced ability to clear the homologous strain of NTHI from the lower respiratory tract. Immunization did not influence the number of phagocytic cells recovered by bronchoalveolar lavage from mice before or after intrapulmonary challenge with NTHI. Immunization also induced the synthesis of relatively large quantities of NTHI-directed antibodies which were detectable in both the bloodstream and the alveolar spaces of the lung. Radioimmunoprecipitation and Western blot (immunoblot) analyses indicated that these antibodies were directed against both the proteins and lipooligosaccharide (LOS) in the NTHI outer membrane. Bactericidal and opsonophagocytic assays determined that the NTHI-directed antibodies in the serum were functional and able to kill or opsonize the homologous NTHI strain. Mice immunized with an NTHI major outer membrane protein-LOS complex also had an increased ability to effect pulmonary clearance of NTHI. Serum and bronchoalveolar lavage fluid collected from these animals immunized with the outer membrane protein-LOS complex contained relatively high levels of antibodies to both of these antigens. The serum from these animals also possessed bactericidal and opsonic activity against the homologous NTHI strain. These results indicate that systemic immunization can enhance the ability of experimental animals to clear NTHI from the lower respiratory tract and suggest that immunoprophylaxis of NTHI pulmonary disease may be feasible.
Similar articles
-
Effect of primary immunization on pulmonary clearance of nontypable Haemophilus influenzae.Am J Respir Cell Mol Biol. 1989 Sep;1(3):201-10. doi: 10.1165/ajrcmb/1.3.201. Am J Respir Cell Mol Biol. 1989. PMID: 2624760
-
Enhanced respiratory clearance of nontypeable Haemophilus influenzae following mucosal immunization with P6 in a rat model.Infect Immun. 1995 Aug;63(8):2931-40. doi: 10.1128/iai.63.8.2931-2940.1995. Infect Immun. 1995. PMID: 7622215 Free PMC article.
-
Antibodies specific for the high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae are opsonophagocytic for both homologous and heterologous strains.Clin Vaccine Immunol. 2006 Dec;13(12):1333-42. doi: 10.1128/CVI.00221-06. Epub 2006 Oct 4. Clin Vaccine Immunol. 2006. PMID: 17021246 Free PMC article.
-
Pulmonary clearance of nontypable Haemophilus influenzae.Pediatr Infect Dis J. 1989 Jan;8(1 Suppl):S69-72. Pediatr Infect Dis J. 1989. PMID: 2648297 Review. No abstract available.
-
Epidemiology and prevention of respiratory tract infections due to nonencapsulated Haemophilus influenzae.J Infect Dis. 1992 Jun;165 Suppl 1:S177-80. doi: 10.1093/infdis/165-supplement_1-s177. J Infect Dis. 1992. PMID: 1588158 Review.
Cited by
-
Haemophilus influenzae responds to glucocorticoids used in asthma therapy by modulation of biofilm formation and antibiotic resistance.EMBO Mol Med. 2015 Aug;7(8):1018-33. doi: 10.15252/emmm.201505088. EMBO Mol Med. 2015. Retraction in: EMBO Mol Med. 2018 Apr;10(4):e70001. doi: 10.15252/emmm.201870001. PMID: 25995336 Free PMC article. Retracted.
-
Release of leukotriene B4 from human neutrophils after interaction with nontypeable Haemophilus influenzae.Infect Immun. 1991 Nov;59(11):4221-6. doi: 10.1128/iai.59.11.4221-4226.1991. Infect Immun. 1991. PMID: 1657784 Free PMC article.
-
Cloning of the gene encoding the major outer membrane protein of Haemophilus influenzae type b.Infect Immun. 1988 Oct;56(10):2709-16. doi: 10.1128/iai.56.10.2709-2716.1988. Infect Immun. 1988. PMID: 3262090 Free PMC article.
-
Bacterial infection in chronic obstructive pulmonary disease in 2000: a state-of-the-art review.Clin Microbiol Rev. 2001 Apr;14(2):336-63. doi: 10.1128/CMR.14.2.336-363.2001. Clin Microbiol Rev. 2001. PMID: 11292642 Free PMC article. Review.
-
Potential of a novel protein, OMP26, from nontypeable Haemophilus influenzae to enhance pulmonary clearance in a rat model.Infect Immun. 1998 May;66(5):2272-8. doi: 10.1128/IAI.66.5.2272-2278.1998. Infect Immun. 1998. PMID: 9573117 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources