doi: 10.1016/0003-2697(88)90365-x.
Detection of tubulin-binding proteins by an overlay assay
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- PMID: 3245580
- DOI: 10.1016/0003-2697(88)90365-x
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Detection of tubulin-binding proteins by an overlay assay
Anal Biochem.
.
Abstract
A blot overlay technique to detect tubulin-binding proteins has been developed. It involves the fractionation of the putative tubulin-binding proteins by gel electrophoresis, their transfer to nitrocellulose paper, and the incubation of the nitrocellulose sheet with purified tubulin. The proteins which bind tubulin are recognized by monoclonal anti-tubulin antibodies. Proteins which associate with microtubules through a direct interaction with tubulin are easily detected by this procedure, which has been tested for both known microtubule-associated proteins and newly reported tubulin-binding proteins.
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