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. 2020 Aug;2(3):100145.
doi: 10.1016/j.ajogmf.2020.100145. Epub 2020 May 18.

Vertical transmission of coronavirus disease 2019: severe acute respiratory syndrome coronavirus 2 RNA on the fetal side of the placenta in pregnancies with coronavirus disease 2019-positive mothers and neonates at birth

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Vertical transmission of coronavirus disease 2019: severe acute respiratory syndrome coronavirus 2 RNA on the fetal side of the placenta in pregnancies with coronavirus disease 2019-positive mothers and neonates at birth

Luisa Patanè et al. Am J Obstet Gynecol MFM. 2020 Aug.
No abstract available

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Figures

Figure 1
Figure 1
COVID-19–positive mother and neonate (case 1) with SARS-CoV-2 antigens seen in the villous syncytiotrophoblasts on the fetal side of the placenta A, Chorionic villi showing chronic intervillositis with macrophages in formalin-fixed, paraffin-embedded placental sections using standard brightfield microscopy at 20× magnification. The tissue sections were stained with hematoxylin and eosin (20×). B, Macrophages in the intervillous spaces highlighted by anti-CD68 immunohistochemistry. C, In situ hybridization for SARS-CoV-2 spike protein mRNA, highlighting the presence of SARS-CoV-2 spike antigens in villous syncytiotrophoblasts. The brown dots are positive signals for the presence of SARS-CoV-2 (indicated by black arrows) inside a syncytiotrophoblast of a chorionic villi cross section. COVID-19, coronavirus 2019; SARS-CoV-2, severe acute respiratory coronavirus 2. Patanè. SARS-CoV-2 vertical transmission. AJOG MFM 2020.
Figure 2
Figure 2
COVID-19–positive mother and neonate (case 2), with SARS-CoV-2 antigens seen in the villous syncytiotrophoblasts on the fetal side of the placenta A, Chorionic villi showing chronic intervillositis with macrophages in formalin-fixed, paraffin-embedded placental sections using standard brightfield microscopy at 20× magnification. The tissue sections were stained with hematoxylin and eosin (20×). B, Macrophages in the intervillous spaces highlighted by anti-CD68 immunohistochemistry. C, In situ hybridization for SARS-CoV-2 spike protein mRNA, highlighting the presence of SARS-CoV-2 spike antigens in villous syncytiotrophoblasts. The brown dots are positive signals for the presence of SARS-CoV-2 (indicated by black arrows) inside a syncytiotrophoblast of a chorionic villi cross section. COVID-19, coronavirus 2019; SARS-CoV-2, severe acute respiratory coronavirus 2. Patanè. SARS-CoV-2 vertical transmission. AJOG MFM 2020.
Figure 3
Figure 3
COVID-19–positive mother with a COVID-19–negative neonate (case 3) showing the control placenta from which the virus is absent A, Normal chorionic villi of formalin-fixed, paraffin-embedded placental sections viewed using a standard brightfield microscope at 20× magnification. The tissue sections were stained with hematoxylin and eosin (20×). B, Normal placental tissues incubated with anti-CD68 antibody. C, In situ hybridization for SARS-CoV-2 spike protein mRNA in the absence of SARS-CoV-2 spike antigens in the villous syncytiotrophoblasts. COVID-19, coronavirus 2019; SARS-CoV-2, severe acute respiratory coronavirus 2. Patanè. SARS-CoV-2 vertical transmission. AJOG MFM 2020.

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