Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2

doi:10.1128/JCM.00776-20

. 2020 Jul 23;58(8):e00776-20.

doi: 10.1128/JCM.00776-20. Print 2020 Jul 23.

Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2

Eloise Williams¹, Katherine Bond¹, Bowen Zhang¹, Mark Putland², Deborah A Williamson^{3

4}

Affiliations

¹ Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia.
² Department of Emergency Medicine, Royal Melbourne Hospital, Melbourne, Australia.
³ Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia deborah.williamson@unimelb.edu.au.
⁴ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

PMID: 32317257
PMCID: PMC7383524
DOI: 10.1128/JCM.00776-20

Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2

Eloise Williams et al. J Clin Microbiol. 2020.

. 2020 Jul 23;58(8):e00776-20.

doi: 10.1128/JCM.00776-20. Print 2020 Jul 23.

Authors

Eloise Williams¹, Katherine Bond¹, Bowen Zhang¹, Mark Putland², Deborah A Williamson^{3

4}

Affiliations

¹ Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia.
² Department of Emergency Medicine, Royal Melbourne Hospital, Melbourne, Australia.
³ Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia deborah.williamson@unimelb.edu.au.
⁴ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

PMID: 32317257
PMCID: PMC7383524
DOI: 10.1128/JCM.00776-20

No abstract available

Keywords: COVID-19; RT-PCR; SARS-CoV-2; clinical microbiology.

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Figures

**FIG 1**
(A) Median cycle threshold (*C_T*) value in nasopharyngeal swabs and saliva specimens positive for SARS-CoV-2. NPS, nasopharyngeal swab. (B) Median cycle threshold (*C_T*) value and days from symptom onset in nasopharyngeal swabs and saliva specimens positive for SARS-CoV-2. Data points represent the median *C_T* value from patient samples, and bars represent the interquartile range. The slope represents the line of best fit.

See this image and copyright information in PMC

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References

1. To KK, Tsang OT, Leung WS, Tam AR, Wu TC, Lung DC, Yip CC, Cai JP, Chan JM, Chik TS, Lau DP, Choi CY, Chen LL, Chan WM, Chan KH, Ip JD, Ng AC, Poon RW, Luo CT, Cheng VC, Chan JF, Hung IF, Chen Z, Chen H, Yuen KY. 2020. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect Dis doi:10.1016/s1473-3099(20)30196-1. - DOI - PMC - PubMed
1. Kim YG, Yun SG, Kim MY, Park K, Cho CH, Yoon SY, Nam MH, Lee CK, Cho YJ, Lim CS. 2017. Comparison between saliva and nasopharyngeal swab specimens for detection of respiratory viruses by multiplex reverse transcription-PCR. J Clin Microbiol 55:226–233. doi:10.1128/JCM.01704-16. - DOI - PMC - PubMed
1. Caly L, Druce J, Roberts J, Bond K, Tran T, Kostecki R, Yoga Y, Naughton W, Taiaroa G, Seemann T, Schultz MB, Howden BP, Korman TM, Lewin SR, Williamson DA, Catton MG. 2020. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust doi:10.5694/mja2.50569. - DOI - PMC - PubMed
1. To KK, Tsang OT, Chik-Yan Yip C, Chan KH, Wu TC, Chan JMC, Leung WS, Chik TS, Choi CY, Kandamby DH, Lung DC, Tam AR, Poon RW, Fung AY, Hung IF, Cheng VC, Chan JF, Yuen KY. 2020. Consistent detection of 2019 novel coronavirus in saliva. Clin Infect Dis doi:10.1093/cid/ciaa149. - DOI - PMC - PubMed
1. Ranney ML, Griffeth V, Jha AK. 2020. Critical supply shortages—the need for ventilators and personal protective equipment during the Covid-19 pandemic. N Engl J Med doi:10.1056/NEJMp2006141. - DOI - PubMed

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[1] To KK, Tsang OT, Leung WS, Tam AR, Wu TC, Lung DC, Yip CC, Cai JP, Chan JM, Chik TS, Lau DP, Choi CY, Chen LL, Chan WM, Chan KH, Ip JD, Ng AC, Poon RW, Luo CT, Cheng VC, Chan JF, Hung IF, Chen Z, Chen H, Yuen KY. 2020. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect Dis doi:10.1016/s1473-3099(20)30196-1. - DOI - PMC - PubMed

[2] To KK, Tsang OT, Leung WS, Tam AR, Wu TC, Lung DC, Yip CC, Cai JP, Chan JM, Chik TS, Lau DP, Choi CY, Chen LL, Chan WM, Chan KH, Ip JD, Ng AC, Poon RW, Luo CT, Cheng VC, Chan JF, Hung IF, Chen Z, Chen H, Yuen KY. 2020. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect Dis doi:10.1016/s1473-3099(20)30196-1. - DOI - PMC - PubMed

[3] Kim YG, Yun SG, Kim MY, Park K, Cho CH, Yoon SY, Nam MH, Lee CK, Cho YJ, Lim CS. 2017. Comparison between saliva and nasopharyngeal swab specimens for detection of respiratory viruses by multiplex reverse transcription-PCR. J Clin Microbiol 55:226–233. doi:10.1128/JCM.01704-16. - DOI - PMC - PubMed

[4] Kim YG, Yun SG, Kim MY, Park K, Cho CH, Yoon SY, Nam MH, Lee CK, Cho YJ, Lim CS. 2017. Comparison between saliva and nasopharyngeal swab specimens for detection of respiratory viruses by multiplex reverse transcription-PCR. J Clin Microbiol 55:226–233. doi:10.1128/JCM.01704-16. - DOI - PMC - PubMed

[5] Caly L, Druce J, Roberts J, Bond K, Tran T, Kostecki R, Yoga Y, Naughton W, Taiaroa G, Seemann T, Schultz MB, Howden BP, Korman TM, Lewin SR, Williamson DA, Catton MG. 2020. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust doi:10.5694/mja2.50569. - DOI - PMC - PubMed

[6] Caly L, Druce J, Roberts J, Bond K, Tran T, Kostecki R, Yoga Y, Naughton W, Taiaroa G, Seemann T, Schultz MB, Howden BP, Korman TM, Lewin SR, Williamson DA, Catton MG. 2020. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust doi:10.5694/mja2.50569. - DOI - PMC - PubMed

[7] To KK, Tsang OT, Chik-Yan Yip C, Chan KH, Wu TC, Chan JMC, Leung WS, Chik TS, Choi CY, Kandamby DH, Lung DC, Tam AR, Poon RW, Fung AY, Hung IF, Cheng VC, Chan JF, Yuen KY. 2020. Consistent detection of 2019 novel coronavirus in saliva. Clin Infect Dis doi:10.1093/cid/ciaa149. - DOI - PMC - PubMed

[8] To KK, Tsang OT, Chik-Yan Yip C, Chan KH, Wu TC, Chan JMC, Leung WS, Chik TS, Choi CY, Kandamby DH, Lung DC, Tam AR, Poon RW, Fung AY, Hung IF, Cheng VC, Chan JF, Yuen KY. 2020. Consistent detection of 2019 novel coronavirus in saliva. Clin Infect Dis doi:10.1093/cid/ciaa149. - DOI - PMC - PubMed

[9] Ranney ML, Griffeth V, Jha AK. 2020. Critical supply shortages—the need for ventilators and personal protective equipment during the Covid-19 pandemic. N Engl J Med doi:10.1056/NEJMp2006141. - DOI - PubMed

[10] Ranney ML, Griffeth V, Jha AK. 2020. Critical supply shortages—the need for ventilators and personal protective equipment during the Covid-19 pandemic. N Engl J Med doi:10.1056/NEJMp2006141. - DOI - PubMed

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Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2

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Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2

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